TY - JOUR
T1 - Removal of Stomatin, a Membrane-Associated Cell Division Protein, Results in Specific Cellular Lipid Changes
AU - Donà, Federico
AU - Özbalci, Cagakan
AU - Paquola, Andrea
AU - Ferrentino, Federica
AU - Terry, Stephen J
AU - Storck, Elisabeth M
AU - Wang, Gaoge
AU - Eggert, Ulrike S
N1 - Funding Information:
This work was funded by European Research Council Consolidator Grant 306659 and Wellcome Trust Investigator Award 110060/Z/15/Z. G.W. was funded by a K-CSC PhD Scholarship.
Publisher Copyright:
© 2022 American Chemical Society.
PY - 2022/10/5
Y1 - 2022/10/5
N2 - Lipids are key constituents of all cells, which express thousands of different lipid species. In most cases, it is not known why cells synthesize such diverse lipidomes, nor what regulates their metabolism. Although it is known that dividing cells specifically regulate their lipid content and that the correct lipid complement is required for successful division, it is unclear how lipids connect with the cell division machinery. Here, we report that the membrane protein stomatin is involved in the cytokinesis step of cell division. Although it is not a lipid biosynthetic enzyme, depletion of stomatin causes cells to change their lipidomes. These changes include specific lipid species, like ether lipids, and lipid families like phosphatidylcholines. Addition of exogenous phosphatidylcholines rescues stomatin-induced defects. These data suggest that stomatin interfaces with lipid metabolism. Stomatin has multiple contacts with the plasma membrane and we identify which sites are required for its role in cell division, as well as associated lipid shifts. We also show that stomatin's mobility on the plasma membrane changes during division, further supporting the requirement for a highly regulated physical interaction between membrane lipids and this newly identified cell division protein.
AB - Lipids are key constituents of all cells, which express thousands of different lipid species. In most cases, it is not known why cells synthesize such diverse lipidomes, nor what regulates their metabolism. Although it is known that dividing cells specifically regulate their lipid content and that the correct lipid complement is required for successful division, it is unclear how lipids connect with the cell division machinery. Here, we report that the membrane protein stomatin is involved in the cytokinesis step of cell division. Although it is not a lipid biosynthetic enzyme, depletion of stomatin causes cells to change their lipidomes. These changes include specific lipid species, like ether lipids, and lipid families like phosphatidylcholines. Addition of exogenous phosphatidylcholines rescues stomatin-induced defects. These data suggest that stomatin interfaces with lipid metabolism. Stomatin has multiple contacts with the plasma membrane and we identify which sites are required for its role in cell division, as well as associated lipid shifts. We also show that stomatin's mobility on the plasma membrane changes during division, further supporting the requirement for a highly regulated physical interaction between membrane lipids and this newly identified cell division protein.
KW - Cell Division
KW - Ethers
KW - Lipid Metabolism
KW - Membrane Lipids
KW - Membrane Proteins/metabolism
KW - Phosphatidylcholines
UR - http://www.scopus.com/inward/record.url?scp=85139042352&partnerID=8YFLogxK
U2 - 10.1021/jacs.2c07907
DO - 10.1021/jacs.2c07907
M3 - Article
C2 - 36136763
SN - 0002-7863
VL - 144
SP - 18069
EP - 18074
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
IS - 39
ER -