Replication-competent retroviral vectors for expressing genes in avian cells in vitro and in vivo.

TY - JOUR

T1 - Replication-competent retroviral vectors for expressing genes in avian cells in vitro and in vivo.

AU - Bell, E

AU - Brickell, P. M.

PY - 1997

Y1 - 1997

N2 - Replication-competent retroviral vectors based on Rous sarcoma virus (RSV) are becoming increasingly popular for expressing genes in both primary cell cultures and embryonic chick tissues in ovo. In this article, we review the features of RSV and its life cycle that make it suitable for use as a vector. We describe the design and use of the RCAS and RCAS (BP) series of vectors, which are currently the most widely used RSV-based vectors, illustrating both their strengths and weakness. Finally, we outline laboratory protocols suitable for the banding of these retroviral vectors.

AB - Replication-competent retroviral vectors based on Rous sarcoma virus (RSV) are becoming increasingly popular for expressing genes in both primary cell cultures and embryonic chick tissues in ovo. In this article, we review the features of RSV and its life cycle that make it suitable for use as a vector. We describe the design and use of the RCAS and RCAS (BP) series of vectors, which are currently the most widely used RSV-based vectors, illustrating both their strengths and weakness. Finally, we outline laboratory protocols suitable for the banding of these retroviral vectors.

M3 - Article

VL - 7

SP - 289

EP - 298

JO - Molecular Biotechnology

JF - Molecular Biotechnology

IS - 3

ER -