Report into a discordant result at D16S539 between SGM Plus® and PowerPlex® ESI 16 kits in a criminal case sample and implications for the UK National DNA Database upgrade

TY - JOUR

T1 - Report into a discordant result at D16S539 between SGM Plus® and PowerPlex® ESI 16 kits in a criminal case sample and implications for the UK National DNA Database upgrade

AU - Revoir, A.

AU - Ballard, D.J.

AU - Syndercombe Court, D.

PY - 2014/1

Y1 - 2014/1

N2 - Upon re-testing of a DNA extract as part of a defence examination, a discordant result was observed at D16S539. Further STR testing and DNA sequencing of the sample identified the cause as a primer binding site mutation which was shown to be a previously unreported SNP. The testing results obtained in this case are considered in light of the current ongoing Multiplex Upgrade Project in the UK and the likely increase in discordant results that may be observed once different next generation kits are introduced.

AB - Upon re-testing of a DNA extract as part of a defence examination, a discordant result was observed at D16S539. Further STR testing and DNA sequencing of the sample identified the cause as a primer binding site mutation which was shown to be a previously unreported SNP. The testing results obtained in this case are considered in light of the current ongoing Multiplex Upgrade Project in the UK and the likely increase in discordant results that may be observed once different next generation kits are introduced.

KW - Multiplex Upgrade Project

KW - Next generation multiplex kits

KW - Discordance

KW - UK National DNA database

KW - D16S539

UR - https://www.scopus.com/pages/publications/84892480709

U2 - 10.1016/j.scijus.2013.08.005

DO - 10.1016/j.scijus.2013.08.005

M3 - Article

SN - 1355-0306

VL - 54

SP - 95

EP - 97

JO - Science and Justice

JF - Science and Justice

IS - 1

ER -