Reporter gene-engineering of human induced pluripotent stem cells during differentiation renders in vivo traceable hepatocyte-like cells accessible

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)
143 Downloads (Pure)

Abstract

Primary hepatocyte transplantation (HTx) is a safe cell therapy for patients with liver disease, but wider application is circumvented by poor cell engraftment due to limitations in hepatocyte quality and transplantation strategies. Hepatocyte-like cells (HLCs) derived from human induced pluripotent stem cells (hiPSC) are considered a promising alternative but also require optimisation of transplantation and are often transplanted prior to full maturation. Whole-body in vivo imaging would be highly beneficial to assess engraftment non-invasively and monitor the transplanted cells in the short and long-term.
Here we report a lentiviral transduction approach designed to engineer hiPSC-derived HLCs during differentiation. This strategy resulted in the successful production of sodium iodide symporter (NIS)-expressing HLCs that were functionally characterised, transplanted into mice, and subsequently imaged using radionuclide tomography.
Original languageEnglish
Article number101599
JournalStem Cell Research
Volume41
Early online date15 Oct 2019
DOIs
Publication statusPublished - 1 Dec 2019

Keywords

  • Cell tracking
  • Hepatocyte-like cells
  • Human sodium iodide symporter
  • Induced pluripotent stem cells
  • Lentivirus
  • Radionuclide imaging

Fingerprint

Dive into the research topics of 'Reporter gene-engineering of human induced pluripotent stem cells during differentiation renders in vivo traceable hepatocyte-like cells accessible'. Together they form a unique fingerprint.

Cite this