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Resveratrol-Induced Vascular Progenitor Differentiation towards Endothelial Lineage via MiR-21/Akt/β-Catenin Is Protective in Vessel Graft Models

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Resveratrol-Induced Vascular Progenitor Differentiation towards Endothelial Lineage via MiR-21/Akt/β-Catenin Is Protective in Vessel Graft Models. / Campagnolo, Paola; Hong, Xuechong; Di Bernardini, Elisabetta; Smyrnias, Ioannis; Hu, Yanhua; Xu, Qingbo; Xiao, Qingzhong (Editor).

In: PL o S One , Vol. 10, No. 5, e0125122, 11.05.2015.

Research output: Contribution to journalArticle

Harvard

Campagnolo, P, Hong, X, Di Bernardini, E, Smyrnias, I, Hu, Y, Xu, Q & Xiao, Q (ed.) 2015, 'Resveratrol-Induced Vascular Progenitor Differentiation towards Endothelial Lineage via MiR-21/Akt/β-Catenin Is Protective in Vessel Graft Models', PL o S One , vol. 10, no. 5, e0125122. https://doi.org/10.1371/journal.pone.0125122

APA

Campagnolo, P., Hong, X., Di Bernardini, E., Smyrnias, I., Hu, Y., Xu, Q., & Xiao, Q. (Ed.) (2015). Resveratrol-Induced Vascular Progenitor Differentiation towards Endothelial Lineage via MiR-21/Akt/β-Catenin Is Protective in Vessel Graft Models. PL o S One , 10(5), [e0125122]. https://doi.org/10.1371/journal.pone.0125122

Vancouver

Campagnolo P, Hong X, Di Bernardini E, Smyrnias I, Hu Y, Xu Q et al. Resveratrol-Induced Vascular Progenitor Differentiation towards Endothelial Lineage via MiR-21/Akt/β-Catenin Is Protective in Vessel Graft Models. PL o S One . 2015 May 11;10(5). e0125122. https://doi.org/10.1371/journal.pone.0125122

Author

Campagnolo, Paola ; Hong, Xuechong ; Di Bernardini, Elisabetta ; Smyrnias, Ioannis ; Hu, Yanhua ; Xu, Qingbo ; Xiao, Qingzhong (Editor). / Resveratrol-Induced Vascular Progenitor Differentiation towards Endothelial Lineage via MiR-21/Akt/β-Catenin Is Protective in Vessel Graft Models. In: PL o S One . 2015 ; Vol. 10, No. 5.

Bibtex Download

@article{16f1fb28191645978782be1c0c4dc9fe,
title = "Resveratrol-Induced Vascular Progenitor Differentiation towards Endothelial Lineage via MiR-21/Akt/β-Catenin Is Protective in Vessel Graft Models",
abstract = "Background and Purpose:Vessel graft failure is typically associated with arteriosclerosis, in which endothelial dysfunction/damage is a key event. Resveratrol has been shown to possess cardioprotective capacity and to reduce atherosclerosis. We aimed to study the influence of resveratrol on the behavior of resident stem cells that may contribute to graft arteriosclerosis.Experimental Approach:Vascular resident progenitor cells and embryonic stem cells were treated with resveratrol under differentiating conditions and endothelial markers expression was evaluated. Expression of miR-21 and β-catenin was also tested and exogenously modified. Effects of resveratrol treatment in an ex vivo re-endothelialization model and on mice undergone vascular graft were evaluated.Key Results:Resveratrol induced expression of endothelial markers such as CD31, VE-cadherin and eNOS in both progenitor and stem cells. We demonstrated that resveratrol significantly reduced miR-21 expression, which in turn reduced Akt phosphorylation. This signal cascade diminished the amount of nuclear β-catenin, inducing endothelial marker expression and increasing tube-like formation by progenitor cells. Both the inhibition of miR-21 and the knockdown of β-catenin were able to recapitulate the effect of resveratrol application. Ex vivo, progenitor cells treated with resveratrol produced better endothelialization of the decellularized vessel. Finally, in a mouse model of vessel graft, a resveratrol-enhanced diet was able to reduce lesion formation.Conclusions and Implications:We provide the first evidence that oral administration of resveratrol can reduce neointimal formation in a model of vascular graft and elucidated the underpinning miR-21/Akt/β-catenin dependent mechanism. These findings may support the beneficial effect of resveratrol supplementation for graft failure prevention.",
author = "Paola Campagnolo and Xuechong Hong and {Di Bernardini}, Elisabetta and Ioannis Smyrnias and Yanhua Hu and Qingbo Xu and Qingzhong Xiao",
year = "2015",
month = may,
day = "11",
doi = "10.1371/journal.pone.0125122",
language = "English",
volume = "10",
journal = "PL o S One ",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "5",

}

RIS (suitable for import to EndNote) Download

TY - JOUR

T1 - Resveratrol-Induced Vascular Progenitor Differentiation towards Endothelial Lineage via MiR-21/Akt/β-Catenin Is Protective in Vessel Graft Models

AU - Campagnolo, Paola

AU - Hong, Xuechong

AU - Di Bernardini, Elisabetta

AU - Smyrnias, Ioannis

AU - Hu, Yanhua

AU - Xu, Qingbo

A2 - Xiao, Qingzhong

PY - 2015/5/11

Y1 - 2015/5/11

N2 - Background and Purpose:Vessel graft failure is typically associated with arteriosclerosis, in which endothelial dysfunction/damage is a key event. Resveratrol has been shown to possess cardioprotective capacity and to reduce atherosclerosis. We aimed to study the influence of resveratrol on the behavior of resident stem cells that may contribute to graft arteriosclerosis.Experimental Approach:Vascular resident progenitor cells and embryonic stem cells were treated with resveratrol under differentiating conditions and endothelial markers expression was evaluated. Expression of miR-21 and β-catenin was also tested and exogenously modified. Effects of resveratrol treatment in an ex vivo re-endothelialization model and on mice undergone vascular graft were evaluated.Key Results:Resveratrol induced expression of endothelial markers such as CD31, VE-cadherin and eNOS in both progenitor and stem cells. We demonstrated that resveratrol significantly reduced miR-21 expression, which in turn reduced Akt phosphorylation. This signal cascade diminished the amount of nuclear β-catenin, inducing endothelial marker expression and increasing tube-like formation by progenitor cells. Both the inhibition of miR-21 and the knockdown of β-catenin were able to recapitulate the effect of resveratrol application. Ex vivo, progenitor cells treated with resveratrol produced better endothelialization of the decellularized vessel. Finally, in a mouse model of vessel graft, a resveratrol-enhanced diet was able to reduce lesion formation.Conclusions and Implications:We provide the first evidence that oral administration of resveratrol can reduce neointimal formation in a model of vascular graft and elucidated the underpinning miR-21/Akt/β-catenin dependent mechanism. These findings may support the beneficial effect of resveratrol supplementation for graft failure prevention.

AB - Background and Purpose:Vessel graft failure is typically associated with arteriosclerosis, in which endothelial dysfunction/damage is a key event. Resveratrol has been shown to possess cardioprotective capacity and to reduce atherosclerosis. We aimed to study the influence of resveratrol on the behavior of resident stem cells that may contribute to graft arteriosclerosis.Experimental Approach:Vascular resident progenitor cells and embryonic stem cells were treated with resveratrol under differentiating conditions and endothelial markers expression was evaluated. Expression of miR-21 and β-catenin was also tested and exogenously modified. Effects of resveratrol treatment in an ex vivo re-endothelialization model and on mice undergone vascular graft were evaluated.Key Results:Resveratrol induced expression of endothelial markers such as CD31, VE-cadherin and eNOS in both progenitor and stem cells. We demonstrated that resveratrol significantly reduced miR-21 expression, which in turn reduced Akt phosphorylation. This signal cascade diminished the amount of nuclear β-catenin, inducing endothelial marker expression and increasing tube-like formation by progenitor cells. Both the inhibition of miR-21 and the knockdown of β-catenin were able to recapitulate the effect of resveratrol application. Ex vivo, progenitor cells treated with resveratrol produced better endothelialization of the decellularized vessel. Finally, in a mouse model of vessel graft, a resveratrol-enhanced diet was able to reduce lesion formation.Conclusions and Implications:We provide the first evidence that oral administration of resveratrol can reduce neointimal formation in a model of vascular graft and elucidated the underpinning miR-21/Akt/β-catenin dependent mechanism. These findings may support the beneficial effect of resveratrol supplementation for graft failure prevention.

U2 - 10.1371/journal.pone.0125122

DO - 10.1371/journal.pone.0125122

M3 - Article

VL - 10

JO - PL o S One

JF - PL o S One

SN - 1932-6203

IS - 5

M1 - e0125122

ER -

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