Retinoic Acid Signaling in B Cells Is Essential for Oral Immunization and Microflora Composition

Eirini Pantazi; Ellen Marks; Emilie Stolarczyk; Nils Lycke; Randolph J. Noelle; Raul Elgueta

doi:10.4049/jimmunol.1500989

Retinoic Acid Signaling in B Cells Is Essential for Oral Immunization and Microflora Composition

Eirini Pantazi, Ellen Marks, Emilie Stolarczyk, Nils Lycke, Randolph J. Noelle, Raul Elgueta

Diabetes

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46 Citations (Scopus)

Abstract

Retinoic acid (RA) is a critical regulator of the intestinal adaptive immune response. However, the intrinsic impact of RA on B cell differentiation in the regulation of gut humoral immunity in vivo has never been directly shown. To address this issue, we have been able to generate a mouse model where B cells specifically express a dominant-negative receptor α for RA. In this study, we show that the silencing of RA signaling in B cells reduces the numbers of IgA(+) Ab-secreting cells both in vitro and in vivo, suggesting that RA has a direct effect on IgA plasma cell differentiation. Moreover, the lack of RA signaling in B cells abrogates Ag-specific IgA responses after oral immunization and affects the microbiota composition. In conclusion, these results suggest that RA signaling in B cells through the RA receptor α is important to generate an effective gut humoral response and to maintain a normal microbiota composition.

Original language	English
Pages (from-to)	1368-1371
Number of pages	4
Journal	Journal of Immunology
Volume	195
Issue number	4
DOIs	https://doi.org/10.4049/jimmunol.1500989
Publication status	Published - 15 Aug 2015

Keywords

Animals
B-Lymphocytes
Cell Differentiation
Gastrointestinal Tract
Gene Expression
Immunization
Immunoglobulin A
Mice
Mice, Transgenic
Microbiota
Plasma Cells
Receptors, Retinoic Acid
Signal Transduction
Tretinoin

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title = "Retinoic Acid Signaling in B Cells Is Essential for Oral Immunization and Microflora Composition",

abstract = "Retinoic acid (RA) is a critical regulator of the intestinal adaptive immune response. However, the intrinsic impact of RA on B cell differentiation in the regulation of gut humoral immunity in vivo has never been directly shown. To address this issue, we have been able to generate a mouse model where B cells specifically express a dominant-negative receptor α for RA. In this study, we show that the silencing of RA signaling in B cells reduces the numbers of IgA(+) Ab-secreting cells both in vitro and in vivo, suggesting that RA has a direct effect on IgA plasma cell differentiation. Moreover, the lack of RA signaling in B cells abrogates Ag-specific IgA responses after oral immunization and affects the microbiota composition. In conclusion, these results suggest that RA signaling in B cells through the RA receptor α is important to generate an effective gut humoral response and to maintain a normal microbiota composition.",

keywords = "Animals, B-Lymphocytes, Cell Differentiation, Gastrointestinal Tract, Gene Expression, Immunization, Immunoglobulin A, Mice, Mice, Transgenic, Microbiota, Plasma Cells, Receptors, Retinoic Acid, Signal Transduction, Tretinoin",

author = "Eirini Pantazi and Ellen Marks and Emilie Stolarczyk and Nils Lycke and Noelle, {Randolph J.} and Raul Elgueta",

note = "Copyright {\textcopyright} 2015 by The American Association of Immunologists, Inc.",

year = "2015",

month = aug,

day = "15",

doi = "10.4049/jimmunol.1500989",

language = "English",

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T1 - Retinoic Acid Signaling in B Cells Is Essential for Oral Immunization and Microflora Composition

AU - Pantazi, Eirini

AU - Marks, Ellen

AU - Stolarczyk, Emilie

AU - Lycke, Nils

AU - Noelle, Randolph J.

AU - Elgueta, Raul

PY - 2015/8/15

Y1 - 2015/8/15

N2 - Retinoic acid (RA) is a critical regulator of the intestinal adaptive immune response. However, the intrinsic impact of RA on B cell differentiation in the regulation of gut humoral immunity in vivo has never been directly shown. To address this issue, we have been able to generate a mouse model where B cells specifically express a dominant-negative receptor α for RA. In this study, we show that the silencing of RA signaling in B cells reduces the numbers of IgA(+) Ab-secreting cells both in vitro and in vivo, suggesting that RA has a direct effect on IgA plasma cell differentiation. Moreover, the lack of RA signaling in B cells abrogates Ag-specific IgA responses after oral immunization and affects the microbiota composition. In conclusion, these results suggest that RA signaling in B cells through the RA receptor α is important to generate an effective gut humoral response and to maintain a normal microbiota composition.

AB - Retinoic acid (RA) is a critical regulator of the intestinal adaptive immune response. However, the intrinsic impact of RA on B cell differentiation in the regulation of gut humoral immunity in vivo has never been directly shown. To address this issue, we have been able to generate a mouse model where B cells specifically express a dominant-negative receptor α for RA. In this study, we show that the silencing of RA signaling in B cells reduces the numbers of IgA(+) Ab-secreting cells both in vitro and in vivo, suggesting that RA has a direct effect on IgA plasma cell differentiation. Moreover, the lack of RA signaling in B cells abrogates Ag-specific IgA responses after oral immunization and affects the microbiota composition. In conclusion, these results suggest that RA signaling in B cells through the RA receptor α is important to generate an effective gut humoral response and to maintain a normal microbiota composition.

KW - Animals

KW - B-Lymphocytes

KW - Cell Differentiation

KW - Gastrointestinal Tract

KW - Gene Expression

KW - Immunization

KW - Immunoglobulin A

KW - Mice

KW - Mice, Transgenic

KW - Microbiota

KW - Plasma Cells

KW - Receptors, Retinoic Acid

KW - Signal Transduction

KW - Tretinoin

U2 - 10.4049/jimmunol.1500989

DO - 10.4049/jimmunol.1500989

M3 - Article

C2 - 26163586

SN - 0022-1767

VL - 195

SP - 1368

EP - 1371

JO - Journal of Immunology

JF - Journal of Immunology

IS - 4

ER -

Retinoic Acid Signaling in B Cells Is Essential for Oral Immunization and Microflora Composition

Abstract

Keywords

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