Revitalising the rudimentary replacement dentition in the mouse

Elena M. Popa, Marcela Buchtova, Abigail S. Tucker

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)
109 Downloads (Pure)


Most mammals have two sets of teeth (diphyodont), a deciduous dentition replaced by a permanent dentition, however the mouse possesses only one tooth generation (monophyodont). In diphyodonts the replacement tooth forms on the lingual side of the first tooth from the successional dental lamina. This lamina expresses the stem/progenitor marker Sox2 and has activated Wnt/B-catenin signalling at its tip. Although the mouse does not replace its teeth a transient rudimentary successional dental lamina (RSDL) still forms during development. The mouse RSDL houses Sox2-positive cells, but no Wnt/B-catenin signalling. Here we show that stabilizing Wnt/B-catenin signaling in the RSDL in the mouse leads to proliferation of the RSDL and formation of lingually positioned teeth. Although Sox2 has been shown to repress Wnt activity, overexpression of Wnts leads to a downregulation of Sox2, suggesting a negative feedback loop in the tooth. In the mouse the first tooth represses the formation of the replacement, and isolation of the RSDL is sufficient to induce formation of a new tooth germ. Our data highlights key mechanisms that may have influenced the evolution of replacement teeth.
Original languageEnglish
Article numberdev171363
Issue number3
Early online date18 Jan 2019
Publication statusPublished - 8 Feb 2019


  • Mammal evolution
  • Minipig
  • Mouse molar
  • Replacement tooth
  • Sox2
  • Wnt/β-catenin


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