Rnd3 interacts with TAO kinases and contributes to mitotic cell rounding and spindle positioning

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)
110 Downloads (Pure)


Rnd3 is an atypical Rho family protein that is constitutively GTP bound, and acts on membranes to induce loss of actin stress fibers and cell rounding. Phosphorylation of Rnd3 promotes 14-3-3 binding and its relocation to the cytosol. Here, we show that Rnd3 binds to the thousand-and-one amino acid kinases TAOK1 and TAOK2 in vitro and in cells. TAOK1 and TAOK2 can phosphorylate serine residues 210, 218 and 240 near the C-terminus of Rnd3, and induce Rnd3 translocation from the plasma membrane to the cytosol. TAOKs are activated catalytically during mitosis and Rnd3 phosphorylation on serine 210 increases in dividing cells. Rnd3 depletion by RNAi inhibits mitotic cell rounding and spindle centralization, and delays breakdown of the intercellular bridge between two daughter cells. Our results show that TAOKs bind, phosphorylate and relocate Rnd3 to the cytosol and that Rnd3 contributes to mitotic cell rounding, spindle positioning and cytokinesis. Rnd3 can therefore participate in the regulation of early and late mitosis and may also act downstream of TAOKs to affect the cytoskeleton.

Original languageEnglish
Article numberjcs235895
JournalJournal of Cell Science
Issue number6
Early online date16 Mar 2020
Publication statusPublished - 16 Mar 2020


  • Kinase and mitosis
  • Rnd3
  • TAOK1
  • TAOK2


Dive into the research topics of 'Rnd3 interacts with TAO kinases and contributes to mitotic cell rounding and spindle positioning'. Together they form a unique fingerprint.

Cite this