Robust Polarity Specification Operates Above a Threshold of Microtubule Dynamicity

Rahul Thadani, Daniel Huang, Snezhana Oliferenko*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Microtubule arrays effect cell polarisation by directing cellular cues for cortical remodelling and growth. Their function depends crucially on the intrinsic dynamic properties of constituent microtubules. Microtubule dynamicity is restricted to a certain range within the confines of a cellular geometry. Thus it is of great interest to determine whether rescaling of dynamic properties of microtubules has consequences for cell polarity. We constructed fission yeast strains exhibiting depressed microtubule dynamics by mutating the beta-tubulin gene, nda3. This interfered with efficient accumulation of a polarity factor Tea1 at cell tips. Interestingly, the polarity machinery in the mutant cells was highly susceptible to perturbations. Simulations of growth zone formation followed by imaging of actin distribution showed a significantly delayed onset of bipolar growth. We propose that there exists a threshold of microtubule dynamicity that allows robust cellular polarisation.

Original languageEnglish
Article numberN/A
Pages (from-to)290-299
Number of pages10
JournalCell Motility and the Cytoskeleton
Volume68
Issue number5
DOIs
Publication statusPublished - May 2011

Keywords

  • microtubule dynamicity
  • cell polarity
  • beta-tubulin
  • Tea1
  • fission yeast
  • FISSION YEAST-CELL
  • GROWTH
  • TEA1P
  • MAINTENANCE
  • CYCLE
  • TIPS

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