ROCK1 and LIMK2 Interact in Spread but Not Blebbing Cancer Cells

TY - JOUR

T1 - ROCK1 and LIMK2 Interact in Spread but Not Blebbing Cancer Cells

AU - Shea, Kerry F.

AU - Wells, Claire M.

AU - Garner, Andrew P.

AU - Jones, Gareth E.

PY - 2008/10/14

Y1 - 2008/10/14

N2 - Cancer cells migrating within a 3D microenvironment are able to adopt either a mesenchymal or amoeboid mode of migration. Amoeboid migration is characterised by membrane blebbing that is dependent on the Rho effectors, ROCK1/2. We identify LIMK2 as the preferred substrate for ROCK1 but find that LIMK2 did not induce membrane blebbing, suggesting that a LIMK2 pathway is not involved in amoeboid-mode migration. In support of this hypothesis, novel FRET data demonstrate a direct interaction between ROCK1 and LIMK2 in polarised but not blebbing cells. Our results point to a specific role for the ROCK1: LIMK2 pathway in mesenchymal-mode migration.

AB - Cancer cells migrating within a 3D microenvironment are able to adopt either a mesenchymal or amoeboid mode of migration. Amoeboid migration is characterised by membrane blebbing that is dependent on the Rho effectors, ROCK1/2. We identify LIMK2 as the preferred substrate for ROCK1 but find that LIMK2 did not induce membrane blebbing, suggesting that a LIMK2 pathway is not involved in amoeboid-mode migration. In support of this hypothesis, novel FRET data demonstrate a direct interaction between ROCK1 and LIMK2 in polarised but not blebbing cells. Our results point to a specific role for the ROCK1: LIMK2 pathway in mesenchymal-mode migration.

M3 - Article

SN - 1932-6203

VL - 3

JO - PL o S One

JF - PL o S One

IS - 10

M1 - e3398

ER -