RTEL1 Regulates G4/R-Loops to Avert Replication-Transcription Collisions

Panagiotis Kotsantis, Sandra Segura-Bayona, Pol Margalef, Paulina Marzec, Phil Ruis, Graeme Hewitt, Roberto Bellelli, Harshil Patel, Robert Goldstone, Anna R. Poetsch, Simon J. Boulton*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)

Abstract

Regulator of telomere length 1 (RTEL1) is an essential helicase that maintains telomere integrity and facilitates DNA replication. The source of replication stress in Rtel1-deficient cells remains unclear. Here, we report that loss of RTEL1 confers extensive transcriptional changes independent of its roles at telomeres. The majority of affected genes in Rtel1−/− cells possess G-quadruplex (G4)-DNA-forming sequences in their promoters and are similarly altered at a transcriptional level in wild-type cells treated with the G4-DNA stabilizer TMPyP4 (5,10,15,20-Tetrakis-(N-methyl-4-pyridyl)porphine). Failure to resolve G4-DNAs formed in the displaced strand of RNA-DNA hybrids in Rtel1−/− cells is suggested by increased R-loops and elevated transcription-replication collisions (TRCs). Moreover, removal of R-loops by RNaseH1 overexpression suppresses TRCs and alleviates the global replication defects observed in Rtel1−/− and Rtel1PIP_box knockin cells and in wild-type cells treated with TMPyP4. We propose that RTEL1 unwinds G4-DNA/R-loops to avert TRCs, which is important to prevent global deregulation in both transcription and DNA replication. Kotsantis et al. report that loss of the helicase RTEL1 leads to extensive transcriptional changes that overlap with those caused by G4 stabilization. Genome-wide replication stress caused by RTEL1 loss, RTEL1/PCNA mutation, and G4 stabilization is associated with R-loop-dependent transcription-replication conflicts.

Original languageEnglish
Article number108546
JournalCell Reports
Volume33
Issue number12
DOIs
Publication statusPublished - 22 Dec 2020

Keywords

  • G-quadruplexes
  • G4-DNA structures
  • genome instability
  • R-loops
  • replication stress
  • RTEL1
  • transcription

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