Scanning total internal reflection fluorescence imaging

A. M. Quirke; S. M. Ameer-Beg; M. Parsons; T. Ng; M. Irving; B. Vojnovic

doi:10.1117/12.648451

Scanning total internal reflection fluorescence imaging

A. M. Quirke^*, S. M. Ameer-Beg, M. Parsons, T. Ng, M. Irving, B. Vojnovic

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference paper › peer-review

1 Citation (Scopus)

Abstract

Cell adhesion and focal complex formation require signalling complexes linking cell adhesion molecules to the cytoskeleton. To understand morphogenetic changes associated with tumour cell spreading, migration and tumour cell metastasis, the molecular mechanisms responsible for the regulation, formation and dissolution at the cell-extracellular matrix (ECM) interface need to be identified. In order to achieve this, an improved axial resolution is desirable. We report on the development of a multi-photon (MP) total internal reflection (TIR) fluorescence lifetime imaging (FLIM) system that allows the selective excitation of fluorophores, with such an improved axial resolution. Results from initial experiments are presented. High excitation efficiency is achieved by the use of a Nikon 1.45 NA TIRF objective using annular illumination.

Original language	English
Title of host publication	Proceedings of SPIE - The International Society for Optical Engineering
Volume	6089
DOIs	https://doi.org/10.1117/12.648451
Publication status	Published - 8 May 2006
Event	Multiphoton Microscopy in the Biomedical Sciences VI - San Jose, CA, United States Duration: 22 Jan 2006 → 24 Jan 2006

Conference

Conference	Multiphoton Microscopy in the Biomedical Sciences VI
Country/Territory	United States
City	San Jose, CA
Period	22/01/2006 → 24/01/2006

Keywords

2-Photon
Axial resolution
ECM
FLIM
Multi-photon
Total internal reflection

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10.1117/12.648451

Cite this

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abstract = "Cell adhesion and focal complex formation require signalling complexes linking cell adhesion molecules to the cytoskeleton. To understand morphogenetic changes associated with tumour cell spreading, migration and tumour cell metastasis, the molecular mechanisms responsible for the regulation, formation and dissolution at the cell-extracellular matrix (ECM) interface need to be identified. In order to achieve this, an improved axial resolution is desirable. We report on the development of a multi-photon (MP) total internal reflection (TIR) fluorescence lifetime imaging (FLIM) system that allows the selective excitation of fluorophores, with such an improved axial resolution. Results from initial experiments are presented. High excitation efficiency is achieved by the use of a Nikon 1.45 NA TIRF objective using annular illumination.",

keywords = "2-Photon, Axial resolution, ECM, FLIM, Multi-photon, Total internal reflection",

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T1 - Scanning total internal reflection fluorescence imaging

AU - Quirke, A. M.

AU - Ameer-Beg, S. M.

AU - Parsons, M.

AU - Ng, T.

AU - Irving, M.

AU - Vojnovic, B.

PY - 2006/5/8

Y1 - 2006/5/8

N2 - Cell adhesion and focal complex formation require signalling complexes linking cell adhesion molecules to the cytoskeleton. To understand morphogenetic changes associated with tumour cell spreading, migration and tumour cell metastasis, the molecular mechanisms responsible for the regulation, formation and dissolution at the cell-extracellular matrix (ECM) interface need to be identified. In order to achieve this, an improved axial resolution is desirable. We report on the development of a multi-photon (MP) total internal reflection (TIR) fluorescence lifetime imaging (FLIM) system that allows the selective excitation of fluorophores, with such an improved axial resolution. Results from initial experiments are presented. High excitation efficiency is achieved by the use of a Nikon 1.45 NA TIRF objective using annular illumination.

AB - Cell adhesion and focal complex formation require signalling complexes linking cell adhesion molecules to the cytoskeleton. To understand morphogenetic changes associated with tumour cell spreading, migration and tumour cell metastasis, the molecular mechanisms responsible for the regulation, formation and dissolution at the cell-extracellular matrix (ECM) interface need to be identified. In order to achieve this, an improved axial resolution is desirable. We report on the development of a multi-photon (MP) total internal reflection (TIR) fluorescence lifetime imaging (FLIM) system that allows the selective excitation of fluorophores, with such an improved axial resolution. Results from initial experiments are presented. High excitation efficiency is achieved by the use of a Nikon 1.45 NA TIRF objective using annular illumination.

KW - 2-Photon

KW - Axial resolution

KW - ECM

KW - FLIM

KW - Multi-photon

KW - Total internal reflection

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DO - 10.1117/12.648451

M3 - Conference paper

AN - SCOPUS:33646192279

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SN - 9780819461315

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BT - Proceedings of SPIE - The International Society for Optical Engineering

T2 - Multiphoton Microscopy in the Biomedical Sciences VI

Y2 - 22 January 2006 through 24 January 2006

ER -

Scanning total internal reflection fluorescence imaging

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Keywords

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