Scanning total internal reflection fluorescence imaging

A. M. Quirke*, S. M. Ameer-Beg, M. Parsons, T. Ng, M. Irving, B. Vojnovic

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference paperpeer-review

1 Citation (Scopus)


Cell adhesion and focal complex formation require signalling complexes linking cell adhesion molecules to the cytoskeleton. To understand morphogenetic changes associated with tumour cell spreading, migration and tumour cell metastasis, the molecular mechanisms responsible for the regulation, formation and dissolution at the cell-extracellular matrix (ECM) interface need to be identified. In order to achieve this, an improved axial resolution is desirable. We report on the development of a multi-photon (MP) total internal reflection (TIR) fluorescence lifetime imaging (FLIM) system that allows the selective excitation of fluorophores, with such an improved axial resolution. Results from initial experiments are presented. High excitation efficiency is achieved by the use of a Nikon 1.45 NA TIRF objective using annular illumination.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
Publication statusPublished - 8 May 2006
EventMultiphoton Microscopy in the Biomedical Sciences VI - San Jose, CA, United States
Duration: 22 Jan 200624 Jan 2006


ConferenceMultiphoton Microscopy in the Biomedical Sciences VI
Country/TerritoryUnited States
CitySan Jose, CA


  • 2-Photon
  • Axial resolution
  • ECM
  • FLIM
  • Multi-photon
  • Total internal reflection


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