Science forum: RIPOSTE: a framework for improving the design and analysis of laboratory-based research.

Nicholas G D Masca; Elizabeth M A Hensor; Victoria R Cornelius; Francesca M Buffa; Helen M Marriott; James M Eales; Michael P Messenger; Amy E Anderson; Chris Boot; Catey Bunce; Robert D Goldin; Jessica Harris; Rod F Hinchliffe; Hiba Junaid; Shaun Kingston; Carmen Martin-Ruiz; Christopher P Nelson; Janet Peacock; Paul T Seed; Bethany Shinkins; Karl J Staples; Jamie Toombs; Adam K A Wright; M Dawn Teare

doi:10.7554/eLife.05519

Science forum: RIPOSTE: a framework for improving the design and analysis of laboratory-based research.

Nicholas G D Masca, Elizabeth M A Hensor, Victoria R Cornelius, Francesca M Buffa, Helen M Marriott, James M Eales, Michael P Messenger, Amy E Anderson, Chris Boot, Catey Bunce, Robert D Goldin, Jessica Harris, Rod F Hinchliffe, Hiba Junaid, Shaun Kingston, Carmen Martin-Ruiz, Christopher P Nelson, Janet Peacock, Paul T Seed, Bethany ShinkinsKarl J Staples, Jamie Toombs, Adam K A Wright, M Dawn Teare

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

21 Citations (Scopus)

Abstract

Lack of reproducibility is an ongoing problem in some areas of the biomedical sciences. Poor experimental design and a failure to engage with experienced statisticians at key stages in the design and analysis of experiments are two factors that contribute to this problem. The RIPOSTE (Reducing IrreProducibility in labOratory STudiEs) framework has been developed to support early and regular discussions between scientists and statisticians in order to improve the design, conduct and analysis of laboratory studies and, therefore, reduce irreproducibility. The framework is intended for using during the early stages of a research project, when specific questions or hypotheses are proposed. The essential points within the framework are explained and illustrated using three examples (a medical equipment test, a macrophage study and a gene expression study). Sound study design minimises the possibility of bias being introduced into experiments and leads to higher quality research with more reproducible results.

Original language	English
Title of host publication	eLife
Pages	e05519
DOIs	https://doi.org/10.7554/eLife.05519
Publication status	Published - 2015

Publication series

Name	eLife
Volume	4

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10.7554/eLife.05519

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Masca, N. G. D., Hensor, E. M. A., Cornelius, V. R., Buffa, F. M., Marriott, H. M., Eales, J. M., Messenger, M. P., Anderson, A. E., Boot, C., Bunce, C., Goldin, R. D., Harris, J., Hinchliffe, R. F., Junaid, H., Kingston, S., Martin-Ruiz, C., Nelson, C. P., Peacock, J., Seed, P. T., ... Teare, M. D. (2015). Science forum: RIPOSTE: a framework for improving the design and analysis of laboratory-based research. In eLife (pp. e05519). (eLife; Vol. 4). https://doi.org/10.7554/eLife.05519

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title = "Science forum: RIPOSTE: a framework for improving the design and analysis of laboratory-based research.",

abstract = "Lack of reproducibility is an ongoing problem in some areas of the biomedical sciences. Poor experimental design and a failure to engage with experienced statisticians at key stages in the design and analysis of experiments are two factors that contribute to this problem. The RIPOSTE (Reducing IrreProducibility in labOratory STudiEs) framework has been developed to support early and regular discussions between scientists and statisticians in order to improve the design, conduct and analysis of laboratory studies and, therefore, reduce irreproducibility. The framework is intended for using during the early stages of a research project, when specific questions or hypotheses are proposed. The essential points within the framework are explained and illustrated using three examples (a medical equipment test, a macrophage study and a gene expression study). Sound study design minimises the possibility of bias being introduced into experiments and leads to higher quality research with more reproducible results.",

author = "Masca, {Nicholas G D} and Hensor, {Elizabeth M A} and Cornelius, {Victoria R} and Buffa, {Francesca M} and Marriott, {Helen M} and Eales, {James M} and Messenger, {Michael P} and Anderson, {Amy E} and Chris Boot and Catey Bunce and Goldin, {Robert D} and Jessica Harris and Hinchliffe, {Rod F} and Hiba Junaid and Shaun Kingston and Carmen Martin-Ruiz and Nelson, {Christopher P} and Janet Peacock and Seed, {Paul T} and Bethany Shinkins and Staples, {Karl J} and Jamie Toombs and Wright, {Adam K A} and Teare, {M Dawn}",

year = "2015",

doi = "10.7554/eLife.05519",

language = "English",

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Masca, NGD, Hensor, EMA, Cornelius, VR, Buffa, FM, Marriott, HM, Eales, JM, Messenger, MP, Anderson, AE, Boot, C, Bunce, C, Goldin, RD, Harris, J, Hinchliffe, RF, Junaid, H, Kingston, S, Martin-Ruiz, C, Nelson, CP, Peacock, J , Seed, PT, Shinkins, B, Staples, KJ, Toombs, J, Wright, AKA & Teare, MD 2015, Science forum: RIPOSTE: a framework for improving the design and analysis of laboratory-based research. in eLife. eLife, vol. 4, pp. e05519. https://doi.org/10.7554/eLife.05519

TY - CHAP

T1 - Science forum: RIPOSTE: a framework for improving the design and analysis of laboratory-based research.

AU - Masca, Nicholas G D

AU - Hensor, Elizabeth M A

AU - Cornelius, Victoria R

AU - Buffa, Francesca M

AU - Marriott, Helen M

AU - Eales, James M

AU - Messenger, Michael P

AU - Anderson, Amy E

AU - Boot, Chris

AU - Bunce, Catey

AU - Goldin, Robert D

AU - Harris, Jessica

AU - Hinchliffe, Rod F

AU - Junaid, Hiba

AU - Kingston, Shaun

AU - Martin-Ruiz, Carmen

AU - Nelson, Christopher P

AU - Peacock, Janet

AU - Seed, Paul T

AU - Shinkins, Bethany

AU - Staples, Karl J

AU - Toombs, Jamie

AU - Wright, Adam K A

AU - Teare, M Dawn

PY - 2015

Y1 - 2015

N2 - Lack of reproducibility is an ongoing problem in some areas of the biomedical sciences. Poor experimental design and a failure to engage with experienced statisticians at key stages in the design and analysis of experiments are two factors that contribute to this problem. The RIPOSTE (Reducing IrreProducibility in labOratory STudiEs) framework has been developed to support early and regular discussions between scientists and statisticians in order to improve the design, conduct and analysis of laboratory studies and, therefore, reduce irreproducibility. The framework is intended for using during the early stages of a research project, when specific questions or hypotheses are proposed. The essential points within the framework are explained and illustrated using three examples (a medical equipment test, a macrophage study and a gene expression study). Sound study design minimises the possibility of bias being introduced into experiments and leads to higher quality research with more reproducible results.

AB - Lack of reproducibility is an ongoing problem in some areas of the biomedical sciences. Poor experimental design and a failure to engage with experienced statisticians at key stages in the design and analysis of experiments are two factors that contribute to this problem. The RIPOSTE (Reducing IrreProducibility in labOratory STudiEs) framework has been developed to support early and regular discussions between scientists and statisticians in order to improve the design, conduct and analysis of laboratory studies and, therefore, reduce irreproducibility. The framework is intended for using during the early stages of a research project, when specific questions or hypotheses are proposed. The essential points within the framework are explained and illustrated using three examples (a medical equipment test, a macrophage study and a gene expression study). Sound study design minimises the possibility of bias being introduced into experiments and leads to higher quality research with more reproducible results.

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DO - 10.7554/eLife.05519

M3 - Chapter

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SN - 2050-084X (Electronic)\r2050-084X (Linking)

T3 - eLife

SP - e05519

BT - eLife

ER -

Science forum: RIPOSTE: a framework for improving the design and analysis of laboratory-based research.

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