Secretion of IFN-gamma and not IL-2 by anergic human T cells correlates with assembly of an immature immune synapse

L M Carlin, K Yanagi, A Verhoef, E N M N Hoen, J Yates, L Gardner, J Lamb, G Lombardi, M J Dallman, D M Davis

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

We report differences in the supramolecular organization of the immunologic synapse (IS) formed by resting and anergic human T cells with agonist peptide-loaded antigen-presenting cells (APCs). T cells reactive to influenza A hemagglutinin peptide or Fel d 1 peptide 4 were rendered both anergic and regulatory by incubation with high doses of agonist peptide in the absence of APCs. At the IS between resting T cells and peptide-loaded APCs, both CD3 epsilon and CD3 zeta initially accumulate within a ring or arc before redistributing within 30 minutes to single or multiple foci more central to the contact. In contrast, at synapses formed by anergized T cells, CD3 epsilon and CD3 zeta remained organized within an arc or ring and failed to redistribute centrally. However, intercellular communication between anergic human T cells and agonist peptide-loaded APCs was not a null event, since it triggered secretion of T-cell interferon gamma (IFN-gamma) but not, for example, inter-leukin 2 (IL-2). Thus, distinct organizations of CD3 at the T-cell IS correlate with different cytokine profiles; the mature IS formed by resting T cells correlates with their production of both IFN-gamma and IL-2, whereas the immature IS formed by anergic T cells seems able to facilitate IFN-gamma but not IL-2 production
Original languageEnglish
Pages (from-to)3874 - 3879
Number of pages6
JournalBlood
Volume106
Issue number12
DOIs
Publication statusPublished - 1 Dec 2005

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