Segregation of telencephalic and eye-field identities inside the zebrafish forebrain territory is controlled by Rx3

C Stigloher, J Ninkovic, M Laplante, A Geling, B Tannhauser, S Topp, H Kikuta, T S Becker, C Houart, L Bally-Cuif

Research output: Contribution to journalArticlepeer-review

82 Citations (Scopus)

Abstract

Anteroposterior patterning of the vertebrate forebrain during gastrulation involves graded Wnt signaling, which segregates anterior fields ( telencephalon and eye) from the diencephalon. How the telencephalic and retinal primordia are subsequently subdivided remains largely unknown. We demonstrate that at late gastrulation the Paired-like homeodomain transcription factor Rx3 biases cell specification choices towards the retinal fate within a population of bipotential precursors of the anterior forebrain: direct cell tracing demonstrates that retinal precursors acquire a telencephalic fate in embryos homozygous for the rx3-null allele ckh(ne2611), characterized by an enlarged telencephalon and a lack of eyes. Chimera analyses further indicate that this function of Rx3 is cell autonomous. Transfating of the eye field in the absence of Rx3 function correlates with a substantial posterior expansion of expression of the Wnt antagonist Tlc and the winged-helix transcription factor Foxg1. These results suggest that the process segregating the telencephalic and eye fields is isolated from diencephalic patterning, and is mediated by Rx3
Original languageEnglish
Pages (from-to)2925 - 2935
Number of pages11
JournalDevelopment (Cambridge): for advances in developmental biology and stem cells
Volume133
Issue number15
DOIs
Publication statusPublished - Aug 2006

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