SF3B1 mutant MDS-initiating cells may arise from the haematopoietic stem cell compartment

Syed A. Mian; Kevin Rouault-Pierre; Alexander E. Smith; Thomas Seidl; Irene Pizzitola; Aytug Kizilors; Austin G. Kulasekararaj; Dominique Bonnet; Ghulam J. Mufti

doi:10.1038/ncomms10004

SF3B1 mutant MDS-initiating cells may arise from the haematopoietic stem cell compartment

Syed A. Mian, Kevin Rouault-Pierre, Alexander E. Smith, Thomas Seidl, Irene Pizzitola, Aytug Kizilors, Austin G. Kulasekararaj, Dominique Bonnet, Ghulam J. Mufti^*

^*Corresponding author for this work

Comprehensive Cancer Centre

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Abstract

Despite the recent evidence of the existence of myelodysplastic syndrome (MDS) stem cells in 5q-MDS patients, it is unclear whether haematopoietic stem cells (HSCs) could also be the initiating cells in other MDS subgroups. Here we demonstrate that SF3B1 mutation(s) in our cohort of MDS patients with ring sideroblasts can arise from CD34 + CD38 - CD45RA - CD90 + CD49f + HSCs and is an initiating event in disease pathogenesis. Xenotransplantation of SF3B1 mutant HSCs leads to persistent long-term engraftment restricted to myeloid lineage. Moreover, genetically diverse evolving subclones of mutant SF3B1 exist in mice, indicating a branching multi-clonal as well as ancestral evolutionary paradigm. Subclonal evolution in mice is also seen in the clinical evolution in patients. Sequential sample analysis shows clonal evolution and selection of the malignant driving clone leading to AML transformation. In conclusion, our data show SF3B1 mutations can propagate from HSCs to myeloid progeny, therefore providing a therapeutic target.

Original language	English
Article number	10004
Number of pages	14
Journal	Nature Communications
Volume	6
Issue number	1
Early online date	8 Dec 2015
DOIs	https://doi.org/10.1038/ncomms10004
Publication status	Published - 8 Dec 2015

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title = "SF3B1 mutant MDS-initiating cells may arise from the haematopoietic stem cell compartment",

abstract = "Despite the recent evidence of the existence of myelodysplastic syndrome (MDS) stem cells in 5q-MDS patients, it is unclear whether haematopoietic stem cells (HSCs) could also be the initiating cells in other MDS subgroups. Here we demonstrate that SF3B1 mutation(s) in our cohort of MDS patients with ring sideroblasts can arise from CD34 + CD38 - CD45RA - CD90 + CD49f + HSCs and is an initiating event in disease pathogenesis. Xenotransplantation of SF3B1 mutant HSCs leads to persistent long-term engraftment restricted to myeloid lineage. Moreover, genetically diverse evolving subclones of mutant SF3B1 exist in mice, indicating a branching multi-clonal as well as ancestral evolutionary paradigm. Subclonal evolution in mice is also seen in the clinical evolution in patients. Sequential sample analysis shows clonal evolution and selection of the malignant driving clone leading to AML transformation. In conclusion, our data show SF3B1 mutations can propagate from HSCs to myeloid progeny, therefore providing a therapeutic target.",

author = "Mian, {Syed A.} and Kevin Rouault-Pierre and Smith, {Alexander E.} and Thomas Seidl and Irene Pizzitola and Aytug Kizilors and Kulasekararaj, {Austin G.} and Dominique Bonnet and Mufti, {Ghulam J.}",

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T1 - SF3B1 mutant MDS-initiating cells may arise from the haematopoietic stem cell compartment

AU - Mian, Syed A.

AU - Rouault-Pierre, Kevin

AU - Smith, Alexander E.

AU - Seidl, Thomas

AU - Pizzitola, Irene

AU - Kizilors, Aytug

AU - Kulasekararaj, Austin G.

AU - Bonnet, Dominique

AU - Mufti, Ghulam J.

PY - 2015/12/8

Y1 - 2015/12/8

N2 - Despite the recent evidence of the existence of myelodysplastic syndrome (MDS) stem cells in 5q-MDS patients, it is unclear whether haematopoietic stem cells (HSCs) could also be the initiating cells in other MDS subgroups. Here we demonstrate that SF3B1 mutation(s) in our cohort of MDS patients with ring sideroblasts can arise from CD34 + CD38 - CD45RA - CD90 + CD49f + HSCs and is an initiating event in disease pathogenesis. Xenotransplantation of SF3B1 mutant HSCs leads to persistent long-term engraftment restricted to myeloid lineage. Moreover, genetically diverse evolving subclones of mutant SF3B1 exist in mice, indicating a branching multi-clonal as well as ancestral evolutionary paradigm. Subclonal evolution in mice is also seen in the clinical evolution in patients. Sequential sample analysis shows clonal evolution and selection of the malignant driving clone leading to AML transformation. In conclusion, our data show SF3B1 mutations can propagate from HSCs to myeloid progeny, therefore providing a therapeutic target.

AB - Despite the recent evidence of the existence of myelodysplastic syndrome (MDS) stem cells in 5q-MDS patients, it is unclear whether haematopoietic stem cells (HSCs) could also be the initiating cells in other MDS subgroups. Here we demonstrate that SF3B1 mutation(s) in our cohort of MDS patients with ring sideroblasts can arise from CD34 + CD38 - CD45RA - CD90 + CD49f + HSCs and is an initiating event in disease pathogenesis. Xenotransplantation of SF3B1 mutant HSCs leads to persistent long-term engraftment restricted to myeloid lineage. Moreover, genetically diverse evolving subclones of mutant SF3B1 exist in mice, indicating a branching multi-clonal as well as ancestral evolutionary paradigm. Subclonal evolution in mice is also seen in the clinical evolution in patients. Sequential sample analysis shows clonal evolution and selection of the malignant driving clone leading to AML transformation. In conclusion, our data show SF3B1 mutations can propagate from HSCs to myeloid progeny, therefore providing a therapeutic target.

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JO - Nature Communications

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SF3B1 mutant MDS-initiating cells may arise from the haematopoietic stem cell compartment

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