TY - CHAP
T1 - Shape analysis and tracking of migrating macrophages
AU - Solis-Lemus, Jose Alonso
AU - Stramer, Brian
AU - Slabaugh, Greg
AU - Reyes-Aldasoro, Constantino Carlos
PY - 2018/5/23
Y1 - 2018/5/23
N2 - Cell migration is important in many human processes of development and disease. In Cancer, migration can be related to metastasis or cell defects. A precise analysis of the cell shapes in biological studies could lead to insights about migration. Therefore, this paper describes an algorithm to iteratively segment, track and analyse the shape of macrophages from fluorescent microscopy image sequences. This process allows observation of shape variations as the cells migrate. The algorithm identifies and separates overlapping and non-overlapping cells, then for the non-overlapping cases analyses the shape and extracts a series of measurements, including the number of 'corner' or pointy edges through a multiscale angle variation matrix, anglegram. The shape evolution algorithm was tested on fluorescently labelled macrophages observed on embryos of Drosophila melanogaster.
AB - Cell migration is important in many human processes of development and disease. In Cancer, migration can be related to metastasis or cell defects. A precise analysis of the cell shapes in biological studies could lead to insights about migration. Therefore, this paper describes an algorithm to iteratively segment, track and analyse the shape of macrophages from fluorescent microscopy image sequences. This process allows observation of shape variations as the cells migrate. The algorithm identifies and separates overlapping and non-overlapping cells, then for the non-overlapping cases analyses the shape and extracts a series of measurements, including the number of 'corner' or pointy edges through a multiscale angle variation matrix, anglegram. The shape evolution algorithm was tested on fluorescently labelled macrophages observed on embryos of Drosophila melanogaster.
KW - Active contours
KW - Shape evolution
UR - http://www.scopus.com/inward/record.url?scp=85048083291&partnerID=8YFLogxK
U2 - 10.1109/ISBI.2018.8363741
DO - 10.1109/ISBI.2018.8363741
M3 - Conference paper
AN - SCOPUS:85048083291
T3 - Proceedings - International Symposium on Biomedical Imaging
SP - 1006
EP - 1009
BT - 2018 IEEE 15th International Symposium on Biomedical Imaging, ISBI 2018
PB - IEEE Computer Society
T2 - 15th IEEE International Symposium on Biomedical Imaging, ISBI 2018
Y2 - 4 April 2018 through 7 April 2018
ER -
