Shape analysis and tracking of migrating macrophages

Jose Alonso Solis-Lemus, Brian Stramer, Greg Slabaugh, Constantino Carlos Reyes-Aldasoro

Research output: Chapter in Book/Report/Conference proceedingConference paperpeer-review

4 Citations (Scopus)


Cell migration is important in many human processes of development and disease. In Cancer, migration can be related to metastasis or cell defects. A precise analysis of the cell shapes in biological studies could lead to insights about migration. Therefore, this paper describes an algorithm to iteratively segment, track and analyse the shape of macrophages from fluorescent microscopy image sequences. This process allows observation of shape variations as the cells migrate. The algorithm identifies and separates overlapping and non-overlapping cells, then for the non-overlapping cases analyses the shape and extracts a series of measurements, including the number of 'corner' or pointy edges through a multiscale angle variation matrix, anglegram. The shape evolution algorithm was tested on fluorescently labelled macrophages observed on embryos of Drosophila melanogaster.

Original languageEnglish
Title of host publication2018 IEEE 15th International Symposium on Biomedical Imaging, ISBI 2018
PublisherIEEE Computer Society
Number of pages4
ISBN (Electronic)9781538636367
Publication statusPublished - 23 May 2018
Event15th IEEE International Symposium on Biomedical Imaging, ISBI 2018 - Washington, United States
Duration: 4 Apr 20187 Apr 2018

Publication series

NameProceedings - International Symposium on Biomedical Imaging
ISSN (Print)1945-7928
ISSN (Electronic)1945-8452


Conference15th IEEE International Symposium on Biomedical Imaging, ISBI 2018
Country/TerritoryUnited States


  • Active contours
  • Shape evolution


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