Sheep CD34+ amniotic fluid cells have hematopoietic potential and engraft after autologous in utero transplantation

S. W.Steven Shaw; Michael P. Blundell; Caterina Pipino; Panicos Shangaris; Panagiotis Maghsoudlou; Durrgah L. Ramachandra; Fanos Georgiades; Michael Boyd; Adrian J. Thrasher; Christopher D. Porada; Graça Almeida-Porada; Po Jen Cheng; Anna L. David; Paolo De Coppi

doi:10.1002/stem.1839

Sheep CD34+ amniotic fluid cells have hematopoietic potential and engraft after autologous in utero transplantation

S. W.Steven Shaw, Michael P. Blundell, Caterina Pipino, Panicos Shangaris, Panagiotis Maghsoudlou, Durrgah L. Ramachandra, Fanos Georgiades, Michael Boyd, Adrian J. Thrasher, Christopher D. Porada, Graça Almeida-Porada, Po Jen Cheng, Anna L. David, Paolo De Coppi^*

^*Corresponding author for this work

Women & Children's Health

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

Unmatched allogeneic in utero stem cell transplantation (IUSCT) produces poor engraftment unless the fetus has congenital immunodeficiency, probably because of maternal and fetal immune responses to injected cells. We studied the functional hematopoietic potential of transduced green fluorescent protein (GFP+) sheep amniotic fluid (AF) stem cells, before and after autologous IUSCT. CD34+ cells were selected from first trimester sheep AF, transduced overnight, and injected intravenously into NOD-SCID-gamma (NSG) mice. At 3 months, primary recipient bone marrow (BM) was injected into secondary NSG recipients. GFP+ cells were detected in the hematopoietic organs and peripheral blood of primary and secondary recipients at 3 months. Autologous IUSCT (transduced GFP+CD34+AF) was performed in fetal sheep. Six months postnatally, lamb BM was injected into secondary NSG recipients. GFP+ cells were detected in the peripheral blood of primary and secondary recipients. This confirms the hematopoietic potential of AF stem cells supporting the concept of autologous IUSCT to treat congenital hematopoietic disease. Stem Cells 2015;33:122-132

Original language	English
Pages (from-to)	122-132
Number of pages	11
Journal	Stem Cells
Volume	33
Issue number	1
Early online date	3 Sept 2014
DOIs	https://doi.org/10.1002/stem.1839
Publication status	Published - 1 Jan 2015

Keywords

Amniotic fluid stem cells
Autologous transplantation
Cell therapy
Sheep CD34

Access to Document

10.1002/stem.1839

Cite this

Shaw, S. W. S., Blundell, M. P., Pipino, C., Shangaris, P., Maghsoudlou, P., Ramachandra, D. L., Georgiades, F., Boyd, M., Thrasher, A. J., Porada, C. D., Almeida-Porada, G., Cheng, P. J., David, A. L., & De Coppi, P. (2015). Sheep CD34+ amniotic fluid cells have hematopoietic potential and engraft after autologous in utero transplantation. Stem Cells, 33(1), 122-132. https://doi.org/10.1002/stem.1839

@article{0f9d8f0a73dc46e29aa83a569885c042,

title = "Sheep CD34+ amniotic fluid cells have hematopoietic potential and engraft after autologous in utero transplantation",

abstract = "Unmatched allogeneic in utero stem cell transplantation (IUSCT) produces poor engraftment unless the fetus has congenital immunodeficiency, probably because of maternal and fetal immune responses to injected cells. We studied the functional hematopoietic potential of transduced green fluorescent protein (GFP+) sheep amniotic fluid (AF) stem cells, before and after autologous IUSCT. CD34+ cells were selected from first trimester sheep AF, transduced overnight, and injected intravenously into NOD-SCID-gamma (NSG) mice. At 3 months, primary recipient bone marrow (BM) was injected into secondary NSG recipients. GFP+ cells were detected in the hematopoietic organs and peripheral blood of primary and secondary recipients at 3 months. Autologous IUSCT (transduced GFP+CD34+AF) was performed in fetal sheep. Six months postnatally, lamb BM was injected into secondary NSG recipients. GFP+ cells were detected in the peripheral blood of primary and secondary recipients. This confirms the hematopoietic potential of AF stem cells supporting the concept of autologous IUSCT to treat congenital hematopoietic disease. Stem Cells 2015;33:122-132",

keywords = "Amniotic fluid stem cells, Autologous transplantation, Cell therapy, Sheep CD34",

author = "Shaw, {S. W.Steven} and Blundell, {Michael P.} and Caterina Pipino and Panicos Shangaris and Panagiotis Maghsoudlou and Ramachandra, {Durrgah L.} and Fanos Georgiades and Michael Boyd and Thrasher, {Adrian J.} and Porada, {Christopher D.} and Gra{\c c}a Almeida-Porada and Cheng, {Po Jen} and David, {Anna L.} and {De Coppi}, Paolo",

year = "2015",

month = jan,

day = "1",

doi = "10.1002/stem.1839",

language = "English",

volume = "33",

pages = "122--132",

journal = "Stem Cells",

issn = "1066-5099",

publisher = "AlphaMed Press",

number = "1",

}

Shaw, SWS, Blundell, MP, Pipino, C, Shangaris, P, Maghsoudlou, P, Ramachandra, DL, Georgiades, F, Boyd, M, Thrasher, AJ, Porada, CD, Almeida-Porada, G, Cheng, PJ, David, AL & De Coppi, P 2015, 'Sheep CD34+ amniotic fluid cells have hematopoietic potential and engraft after autologous in utero transplantation', Stem Cells, vol. 33, no. 1, pp. 122-132. https://doi.org/10.1002/stem.1839

TY - JOUR

T1 - Sheep CD34+ amniotic fluid cells have hematopoietic potential and engraft after autologous in utero transplantation

AU - Shaw, S. W.Steven

AU - Blundell, Michael P.

AU - Pipino, Caterina

AU - Shangaris, Panicos

AU - Maghsoudlou, Panagiotis

AU - Ramachandra, Durrgah L.

AU - Georgiades, Fanos

AU - Boyd, Michael

AU - Thrasher, Adrian J.

AU - Porada, Christopher D.

AU - Almeida-Porada, Graça

AU - Cheng, Po Jen

AU - David, Anna L.

AU - De Coppi, Paolo

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Unmatched allogeneic in utero stem cell transplantation (IUSCT) produces poor engraftment unless the fetus has congenital immunodeficiency, probably because of maternal and fetal immune responses to injected cells. We studied the functional hematopoietic potential of transduced green fluorescent protein (GFP+) sheep amniotic fluid (AF) stem cells, before and after autologous IUSCT. CD34+ cells were selected from first trimester sheep AF, transduced overnight, and injected intravenously into NOD-SCID-gamma (NSG) mice. At 3 months, primary recipient bone marrow (BM) was injected into secondary NSG recipients. GFP+ cells were detected in the hematopoietic organs and peripheral blood of primary and secondary recipients at 3 months. Autologous IUSCT (transduced GFP+CD34+AF) was performed in fetal sheep. Six months postnatally, lamb BM was injected into secondary NSG recipients. GFP+ cells were detected in the peripheral blood of primary and secondary recipients. This confirms the hematopoietic potential of AF stem cells supporting the concept of autologous IUSCT to treat congenital hematopoietic disease. Stem Cells 2015;33:122-132

AB - Unmatched allogeneic in utero stem cell transplantation (IUSCT) produces poor engraftment unless the fetus has congenital immunodeficiency, probably because of maternal and fetal immune responses to injected cells. We studied the functional hematopoietic potential of transduced green fluorescent protein (GFP+) sheep amniotic fluid (AF) stem cells, before and after autologous IUSCT. CD34+ cells were selected from first trimester sheep AF, transduced overnight, and injected intravenously into NOD-SCID-gamma (NSG) mice. At 3 months, primary recipient bone marrow (BM) was injected into secondary NSG recipients. GFP+ cells were detected in the hematopoietic organs and peripheral blood of primary and secondary recipients at 3 months. Autologous IUSCT (transduced GFP+CD34+AF) was performed in fetal sheep. Six months postnatally, lamb BM was injected into secondary NSG recipients. GFP+ cells were detected in the peripheral blood of primary and secondary recipients. This confirms the hematopoietic potential of AF stem cells supporting the concept of autologous IUSCT to treat congenital hematopoietic disease. Stem Cells 2015;33:122-132

KW - Amniotic fluid stem cells

KW - Autologous transplantation

KW - Cell therapy

KW - Sheep CD34

UR - http://www.scopus.com/inward/record.url?scp=84919414285&partnerID=8YFLogxK

U2 - 10.1002/stem.1839

DO - 10.1002/stem.1839

M3 - Article

C2 - 25186828

AN - SCOPUS:84919414285

SN - 1066-5099

VL - 33

SP - 122

EP - 132

JO - Stem Cells

JF - Stem Cells

IS - 1

ER -

Sheep CD34+ amniotic fluid cells have hematopoietic potential and engraft after autologous in utero transplantation

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this