@article{005071b83bfc4b1c979da1414695b891,
title = "Shielding of actin by the endoplasmic reticulum impacts nuclear positioning",
abstract = "Nuclear position is central to cell polarization, and its disruption is associated with various pathologies. The nucleus is moved away from the leading edge of migrating cells through its connection to moving dorsal actin cables, and the absence of connections to immobile ventral stress fibers. It is unclear how these asymmetric nucleo-cytoskeleton connections are established. Here, using an in vitro wound assay, we find that remodeling of endoplasmic reticulum (ER) impacts nuclear positioning through the formation of a barrier that shields immobile ventral stress fibers. The remodeling of ER and perinuclear ER accumulation is mediated by the ER shaping protein Climp-63. Furthermore, ectopic recruitment of the ER to stress fibers restores nuclear positioning in the absence of Climp-63. Our findings suggest that the ER mediates asymmetric nucleo-cytoskeleton connections to position the nucleus.",
author = "Janota, {C{\'a}tia Silva} and Andreia Pinto and Anna Pezzarossa and Pedro Machado and Judite Costa and Pedro Campinho and Franco, {Cl{\'a}udio A.} and Gomes, {Edgar R.}",
note = "Funding Information: We thank Henry Higgs, Andrew Moore, Alexander Palazzo and Gregg Gundersen for reagents. We acknowledge all members of Edgar Gomes, Cl{\'a}udio Franco and Vanessa Morais laboratories, as well as Bruno Cadot, S{\'e}rgio Dias, and Florence Janody for discussions and support. We thank the support from all iMM community. We thank the following iMM facilities; Bioimaging, Comparative Pathology, Flow Cytometry, Rodent and Zebrafish. We thank the Electron Microscopy Core Facility at EMBL, especially to Yannick Schwab. We thank Guy Riddihough (Life Science Editors) for helping with editing the manuscript. We thank Nikon Instruments for loan of equipment. This work was supported by the European Research Council H2020-GA 810207-ARPCOMPLEXITY and FP7-GA 617676 PHONICS (ERG), EMBO installation (ERG), Marine Biological Laboratory Whitman Center Fellowship (ERG), Association pour la Recherche sur le Cancer and La Ligue Nacionale contre le Cancer (ERG) and Funda{\c c}{\~a}o para a Ci{\^e}ncia e a Tecnologia (SFRH/BD/112286/2015)(CJ), European Research Council H2020-GA 679368 AXIAL.EC (CF); Funda{\c c}{\~a}o para a Ci{\^e}ncia e Tecnologia (PTDC/MED-PAT/31639/2017; CEECIND/04251/2017)(CF) and Fondation LeDucq (17CVD03)(CF). Funding Information: We thank Henry Higgs, Andrew Moore, Alexander Palazzo and Gregg Gundersen for reagents. We acknowledge all members of Edgar Gomes, Cl{\'a}udio Franco and Vanessa Morais laboratories, as well as Bruno Cadot, S{\'e}rgio Dias, and Florence Janody for discussions and support. We thank the support from all iMM community. We thank the following iMM facilities; Bioimaging, Comparative Pathology, Flow Cytometry, Rodent and Zebrafish. We thank the Electron Microscopy Core Facility at EMBL, especially to Yannick Schwab. We thank Guy Riddihough (Life Science Editors) for helping with editing the manuscript. We thank Nikon Instruments for loan of equipment. This work was supported by the European Research Council H2020-GA 810207-ARPCOMPLEXITY and FP7-GA 617676 PHONICS (ERG), EMBO installation (ERG), Marine Biological Laboratory Whitman Center Fellowship (ERG), Association pour la Recherche sur le Cancer and La Ligue Nacionale contre le Cancer (ERG) and Funda{\c c}{\~a}o para a Ci{\^e}ncia e a Tecnologia (SFRH/BD/112286/2015)(CJ), European Research Council H2020-GA 679368 AXIAL.EC (CF); Funda{\c c}{\~a}o para a Ci{\^e}ncia e Tecnologia (PTDC/MED-PAT/31639/2017; CEECIND/04251/2017)(CF) and Fondation LeDucq (17CVD03)(CF). Publisher Copyright: {\textcopyright} 2022, The Author(s).",
year = "2022",
month = dec,
doi = "10.1038/s41467-022-30388-3",
language = "English",
volume = "13",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",
number = "1",
}