TY - JOUR
T1 - Single Application Fluorides On Enamel Erosion And Erosion-Abrasion In Vitro
AU - Austin, Rupert
AU - Stenhagen, Kjersti
AU - Hove, Lene
AU - Dunne, Stephen
AU - Moazzez, Rebecca
AU - Bartlett, David
AU - Tveit, Anne
PY - 2011
Y1 - 2011
N2 - Paper #145854Single application fluorides on enamel erosion and erosion-abrasion in vitroR. AUSTIN1, K. STENHAGEN2, L.H. HYSTAD2, S. DUNNE1, R. MOAZZEZ3, D. BARTLETT4, and A.B. TVEIT2, 1Primary Dental Care, King's College London Dental Institute, London, United Kingdom, 2University of Oslo, Oslo, Norway, 3Conservative Dentistry, King's College London Dental Institute, London, United Kingdom, 4Department of Prosthodontics, King's College London Dental Institute, London, United KingdomAbstract OBJECTIVES: To investigate the effect of concentrated fluoride applications on enamel after erosion or erosion-abrasion in vitro.METHODS: Human enamel specimens were pretreated with aqueous fluoride solutions [NaF (0.5M pH 8.0); SnF2 (0.5M pH 2.6); TiF4 (0.5M pH 1.2)] or fluoride varnish [NaF/ CaF2, 22600ppm fluoride (Bifluoride 10®, Voco Gmbh, Cuxhaven, Germany)] before 9 cycles of either cyclic erosion [1 cycle = 2 min agitated immersion in 50ml 0.01M pH2.2 HCl; followed by 60 min immersion in artificial saliva] or cyclic erosion-abrasion [1 cycle = as above; followed by 120 medium soft toothbrush strokes at 200g load in a 1:3 (w/v) slurry of non-fluoridated toothpaste (Kingfisher Natural Toothpaste, Norwich, UK) and artificial saliva. Surface effects were investigated after fluoride pre-treatment; after erosion and after erosion-abrasion qualitatively using scanning electron microscopy (SEM) and quantitatively using energy-dispersive X-ray spectroscopy (EDS) to measure elemental composition (wt %) of the enamel surface.RESULTS: SEM of the enamel surfaces treated with NaF, SnF2 and the varnish showed visible etched enamel prisms present after erosion. Following the erosion-abrasion a smear layer was present for NaF and the varnish. For TiF4, the SEM showed the presence of a coating on the enamel surface following erosion which was removed completely after erosion-abrasion. EDS elemental analysis of the TiF4 treated surface found 7% Ti remaining after erosion vs. 16% at pre-treatment, however all other fluoride preparations were removed after erosion and erosion-abrasion except for 1% Na remaining after erosion-abrasion for the varnish group.CONCLUSION: Following erosion, a TiF4 coating was observed with the SEM and this was supported by the EDS elemental analysis. Following erosion/abrasion, the SEM showed no visible surface effects from any fluoride preparation and this too was supported by EDS analysis.
AB - Paper #145854Single application fluorides on enamel erosion and erosion-abrasion in vitroR. AUSTIN1, K. STENHAGEN2, L.H. HYSTAD2, S. DUNNE1, R. MOAZZEZ3, D. BARTLETT4, and A.B. TVEIT2, 1Primary Dental Care, King's College London Dental Institute, London, United Kingdom, 2University of Oslo, Oslo, Norway, 3Conservative Dentistry, King's College London Dental Institute, London, United Kingdom, 4Department of Prosthodontics, King's College London Dental Institute, London, United KingdomAbstract OBJECTIVES: To investigate the effect of concentrated fluoride applications on enamel after erosion or erosion-abrasion in vitro.METHODS: Human enamel specimens were pretreated with aqueous fluoride solutions [NaF (0.5M pH 8.0); SnF2 (0.5M pH 2.6); TiF4 (0.5M pH 1.2)] or fluoride varnish [NaF/ CaF2, 22600ppm fluoride (Bifluoride 10®, Voco Gmbh, Cuxhaven, Germany)] before 9 cycles of either cyclic erosion [1 cycle = 2 min agitated immersion in 50ml 0.01M pH2.2 HCl; followed by 60 min immersion in artificial saliva] or cyclic erosion-abrasion [1 cycle = as above; followed by 120 medium soft toothbrush strokes at 200g load in a 1:3 (w/v) slurry of non-fluoridated toothpaste (Kingfisher Natural Toothpaste, Norwich, UK) and artificial saliva. Surface effects were investigated after fluoride pre-treatment; after erosion and after erosion-abrasion qualitatively using scanning electron microscopy (SEM) and quantitatively using energy-dispersive X-ray spectroscopy (EDS) to measure elemental composition (wt %) of the enamel surface.RESULTS: SEM of the enamel surfaces treated with NaF, SnF2 and the varnish showed visible etched enamel prisms present after erosion. Following the erosion-abrasion a smear layer was present for NaF and the varnish. For TiF4, the SEM showed the presence of a coating on the enamel surface following erosion which was removed completely after erosion-abrasion. EDS elemental analysis of the TiF4 treated surface found 7% Ti remaining after erosion vs. 16% at pre-treatment, however all other fluoride preparations were removed after erosion and erosion-abrasion except for 1% Na remaining after erosion-abrasion for the varnish group.CONCLUSION: Following erosion, a TiF4 coating was observed with the SEM and this was supported by the EDS elemental analysis. Following erosion/abrasion, the SEM showed no visible surface effects from any fluoride preparation and this too was supported by EDS analysis.
M3 - Meeting abstract
SN - 0022-0345
VL - 90
JO - Journal of Dental Research
JF - Journal of Dental Research
IS - A
ER -