Single-cell gene expression profiling reveals functional heterogeneity of undifferentiated human epidermal cells

David W M Tan, Kim B Jensen, Matthew W B Trotter, John T Connelly, Simon Broad, Fiona M Watt

Research output: Contribution to journalArticlepeer-review

76 Citations (Scopus)

Abstract

Human epidermal stem cells express high levels of beta 1 integrins, delta-like 1 (DLL1) and the EGFR antagonist LRIG1. However, there is cell-to-cell variation in the relative abundance of DLL1 and LRIG1 mRNA transcripts. Single-cell global gene expression profiling showed that undifferentiated cells fell into two clusters delineated by expression of DLL1 and its binding partner syntenin. The DLL1(+) cluster had elevated expression of genes associated with endocytosis, integrin-mediated adhesion and receptor tyrosine kinase signalling. Differentially expressed genes were not independently regulated, as overexpression of DLL1 alone or together with LRIG1 led to the upregulation of other genes in the DLL1(+) cluster. Overexpression of DLL1 and LRIG1 resulted in enhanced extracellular matrix adhesion and increased caveolin-dependent EGFR endocytosis. Further characterisation of CD46, one of the genes upregulated in the DLL1(+) cluster, revealed it to be a novel cell surface marker of human epidermal stem cells. Cells with high endogenous levels of CD46 expressed high levels of beta 1 integrin and DLL1 and were highly adhesive and clonogenic. Knockdown of CD46 decreased proliferative potential and beta 1 integrin-mediated adhesion. Thus, the previously unknown heterogeneity revealed by our studies results in differences in the interaction of undifferentiated basal keratinocytes with their environment.

Original languageEnglish
Article numberN/A
Pages (from-to)1433-1444
Number of pages12
JournalDevelopment
Volume140
Issue number7
DOIs
Publication statusPublished - 1 Apr 2013

Keywords

  • Adhesion
  • CD46
  • Delta
  • Endocytosis
  • MEMBRANE COFACTOR PROTEIN
  • OLIGONUCLEOTIDE MICROARRAY ANALYSIS
  • TRANSIT-AMPLIFYING CELLS
  • GROWTH-FACTOR RECEPTOR
  • STEM-CELLS
  • TERMINAL DIFFERENTIATION
  • CDNA AMPLIFICATION
  • PROGENITOR CELLS
  • C-CBL
  • NOTCH

Fingerprint

Dive into the research topics of 'Single-cell gene expression profiling reveals functional heterogeneity of undifferentiated human epidermal cells'. Together they form a unique fingerprint.

Cite this