Single-cell live imaging

Toru Hiratsuka; Naoki Komatsu

doi:10.1007/978-1-4939-9240-9_24

Single-cell live imaging

Toru Hiratsuka^*, Naoki Komatsu

^*Corresponding author for this work

Centre for Stem Cells & Regenerative Medicine

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

3 Citations (Scopus)

Abstract

Recent fluorescence microscopy allows for high-throughput acquisition of 5D (X, Y, Z, T, and Color) images in various targets such as cultured cells, 3D spheroid/organoid, and even living tissue with single-cell resolution. The technology is considered promising to augment insights on heterogeneous features of both physiological and pathological cell phenotypes, for instance, distinct responses of cancer cells to anticancer drug treatment. Here we overview microscopic applications to capture live cell events for different types of targets, together with a couple of proof of concepts. The 2D live imaging will be exemplified by a FRET-based time-lapse cultured cell imaging, and 3D tissue imaging protocol will be complemented with a method for mouse skin live imaging.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc
Pages	409-421
Number of pages	13
Volume	1979
DOIs	https://doi.org/10.1007/978-1-4939-9240-9_24
Publication status	Published - 1 Jan 2019

Publication series

Name	Methods in Molecular Biology
Volume	1979
ISSN (Print)	1064-3745

Keywords

2D/3D-cultured cell observation
Fluorescent probes
Living mouse observation
Microscopy
Single cell imaging

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/978-1-4939-9240-9_24

Cite this

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AB - Recent fluorescence microscopy allows for high-throughput acquisition of 5D (X, Y, Z, T, and Color) images in various targets such as cultured cells, 3D spheroid/organoid, and even living tissue with single-cell resolution. The technology is considered promising to augment insights on heterogeneous features of both physiological and pathological cell phenotypes, for instance, distinct responses of cancer cells to anticancer drug treatment. Here we overview microscopic applications to capture live cell events for different types of targets, together with a couple of proof of concepts. The 2D live imaging will be exemplified by a FRET-based time-lapse cultured cell imaging, and 3D tissue imaging protocol will be complemented with a method for mouse skin live imaging.

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KW - Fluorescent probes

KW - Living mouse observation

KW - Microscopy

KW - Single cell imaging

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Single-cell live imaging

Abstract

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Keywords

UN SDGs

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