TY - JOUR
T1 - Single-cell transcriptomics in human skin research
T2 - available technologies, technical considerations and disease applications
AU - Theocharidis, Georgios
AU - Tekkela, Stavroula
AU - Veves, Aristidis
AU - McGrath, John A.
AU - Onoufriadis, Alexandros
N1 - Funding Information:
Original studies using single‐cell transcriptomics by the senior author and colleagues have been supported by funding from EBRP. This work was supported by the National Institute for Health Research Biomedical Research Centre based at Guy's and St Thomas’ NHS Foundation Trust and King's College London and by the National Rongxiang Xu Foundation at Harvard Medical School
Funding Information:
Original studies using single‐cell transcriptomics by the senior author and colleagues have been supported by funding from EB Research Partnership. This work was supported by the UK National Institute for Health Research (NIHR) comprehensive Biomedical Research Centre (BRC) award to Guy’s and St. Thomas’ NHS Foundation Trust, in partnership with the King’s College London and King’s College Hospital NHS Foundation Trust and by the National Rongxiang Xu Foundation at Harvard Medical School.
Funding Information:
Original studies using single-cell transcriptomics by the senior author and colleagues have been supported by funding from EBRP. This work was supported by the National Institute for Health Research Biomedical Research Centre based at Guy's and St Thomas? NHS Foundation Trust and King's College London and by the National Rongxiang Xu Foundation at Harvard Medical School Original studies using single-cell transcriptomics by the senior author and colleagues have been supported by funding from EB Research Partnership. This work was supported by the UK National Institute for Health Research (NIHR) comprehensive Biomedical Research Centre (BRC) award to Guy?s and St. Thomas? NHS Foundation Trust, in partnership with the King?s College London and King?s College Hospital NHS Foundation Trust and by the National Rongxiang Xu Foundation at Harvard Medical School.
Publisher Copyright:
© 2022 The Authors. Experimental Dermatology published by John Wiley & Sons Ltd.
PY - 2022/5
Y1 - 2022/5
N2 - Single-cell technologies have revolutionized research in the last decade, including for skin biology. Single-cell RNA sequencing has emerged as a powerful tool allowing the dissection of human disease pathophysiology at unprecedented resolution by assessing cell-to-cell variation, facilitating identification of rare cell populations and elucidating cellular heterogeneity. In dermatology, this technology has been widely applied to inflammatory skin disorders, fibrotic skin diseases, wound healing complications and cutaneous neoplasms. Here, we discuss the available technologies and technical considerations of single-cell RNA sequencing and describe its applications to a broad spectrum of dermatological diseases.
AB - Single-cell technologies have revolutionized research in the last decade, including for skin biology. Single-cell RNA sequencing has emerged as a powerful tool allowing the dissection of human disease pathophysiology at unprecedented resolution by assessing cell-to-cell variation, facilitating identification of rare cell populations and elucidating cellular heterogeneity. In dermatology, this technology has been widely applied to inflammatory skin disorders, fibrotic skin diseases, wound healing complications and cutaneous neoplasms. Here, we discuss the available technologies and technical considerations of single-cell RNA sequencing and describe its applications to a broad spectrum of dermatological diseases.
KW - scRNA-seq
KW - single-cell sequencing
KW - single-cell transcriptomics
KW - skin research
UR - http://www.scopus.com/inward/record.url?scp=85125569673&partnerID=8YFLogxK
U2 - 10.1111/exd.14547
DO - 10.1111/exd.14547
M3 - Review article
C2 - 35196402
AN - SCOPUS:85125569673
SN - 0906-6705
VL - 31
SP - 655
EP - 673
JO - Experimental Dermatology
JF - Experimental Dermatology
IS - 5
ER -
