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Skeletal muscle regeneration in zebrafish

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Original languageEnglish
Title of host publicationMethods in Molecular Biology
EditorsAtsushi Asakura
PublisherHumana Press Inc
Accepted/In press4 Dec 2020

King's Authors

Abstract

Muscle regeneration models have revealed mechanisms of inflammation, wound clearance and stem cell directed repair of damage, thereby informing therapy. Whereas studies of muscle repair are most advanced in rodents, the zebrafish is emerging as an additional model organism with genetic and optical advantages. Various muscle wounding protocols (both chemical and physical) have been published. Here we describe simple, cheap, precise, adaptable and effective wouding protocols and analysis methods for two stages of a larval zebrafish muscle regeneration model. We show examples of how muscle damage, ingression of muscle stem cells, immune cells and regeneration of fibres can be monitored over an extended timecourse in individual larvae. Such analyses have the potential greatly to enhance understanding, by reducing the need to average regeneration responses across indivudals subjected to an unavoidably variable wound stimulus.

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