Slice-illuminated optical projection tomography

TY - JOUR

T1 - Slice-illuminated optical projection tomography

AU - Davis, Samuel P.X.

AU - Wisniewski, Laura

AU - Kumar, Sunil

AU - Correia, Teresa

AU - Arridge, Simon R.

AU - Frankel, Paul

AU - McGinty, James

AU - French, Paul M.W.

PY - 2018/11/15

Y1 - 2018/11/15

N2 - To improve the imaging performance of optical projection tomography (OPT) in live samples, we have explored a parallelized implementation of semi-confocal line illumination and detection to discriminate against scattered photons. Slice-illuminated OPT (sl-OPT) improves reconstruction quality in scattering samples by reducing interpixel crosstalk at the cost of increased acquisition time. For in vivo imaging, this can be ameliorated through the use of compressed sensing on angularly undersampled OPT data sets. Here, we demonstrate sl-OPT applied to 3D imaging of bead phantoms and live adult zebrafish.

AB - To improve the imaging performance of optical projection tomography (OPT) in live samples, we have explored a parallelized implementation of semi-confocal line illumination and detection to discriminate against scattered photons. Slice-illuminated OPT (sl-OPT) improves reconstruction quality in scattering samples by reducing interpixel crosstalk at the cost of increased acquisition time. For in vivo imaging, this can be ameliorated through the use of compressed sensing on angularly undersampled OPT data sets. Here, we demonstrate sl-OPT applied to 3D imaging of bead phantoms and live adult zebrafish.

UR - http://www.scopus.com/inward/record.url?scp=85056446109&partnerID=8YFLogxK

U2 - 10.1364/OL.43.005555

DO - 10.1364/OL.43.005555

M3 - Article

C2 - 30439894

AN - SCOPUS:85056446109

SN - 0146-9592

VL - 43

SP - 5555

EP - 5558

JO - Optics Letters

JF - Optics Letters

IS - 22

ER -