Small molecule screening by imaging

TY - JOUR

T1 - Small molecule screening by imaging

AU - Eggert, Ulrike S

AU - Eggert, Ulrike

AU - Mitchison, Timothy J

PY - 2006/6

Y1 - 2006/6

N2 - Useful small molecule tools can be discovered in imaging screens that measure phenotype in single cells or small organisms. Recent examples include identification of small molecule inhibitors of processes such as cell migration, cytokinesis, mitotic spindle length determination, melanogenesis, aggresome formation, membrane transport and nuclear export. Imaging screens are currently limited by challenges in the areas of image analysis and target identification. We discuss the use of model organisms such as zebrafish in screens and review different methods of target identification. The emerging field of automated image analysis is also introduced.

AB - Useful small molecule tools can be discovered in imaging screens that measure phenotype in single cells or small organisms. Recent examples include identification of small molecule inhibitors of processes such as cell migration, cytokinesis, mitotic spindle length determination, melanogenesis, aggresome formation, membrane transport and nuclear export. Imaging screens are currently limited by challenges in the areas of image analysis and target identification. We discuss the use of model organisms such as zebrafish in screens and review different methods of target identification. The emerging field of automated image analysis is also introduced.

KW - Animals

KW - Drug Evaluation, Preclinical

KW - Humans

KW - Image Processing, Computer-Assisted

U2 - 10.1016/j.cbpa.2006.04.010

DO - 10.1016/j.cbpa.2006.04.010

M3 - Article

C2 - 16682248

SN - 1367-5931

VL - 10

SP - 232

EP - 237

JO - CURRENT OPINION IN CHEMICAL BIOLOGY

JF - CURRENT OPINION IN CHEMICAL BIOLOGY

IS - 3

ER -