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Soft optically-tuneable fluorescence phantoms based on gel wax and quantum dots: a tissue surrogate for fluorescence imaging validation

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Soft optically-tuneable fluorescence phantoms based on gel wax and quantum dots: a tissue surrogate for fluorescence imaging validation. / Xie, Yijing; Maneas, Efthymios; Islam, Shahraz; Peveler, William; Shapey, Jonathan; Xia, Wenfeng; Ourselin, Sebastien; Parkin, Ivan P.; Desjardins, Adrien E.; Vercauteren, Tom.

2019. (Proceedings of SPIE).

Research output: Book/ReportBook

Harvard

Xie, Y, Maneas, E, Islam, S, Peveler, W, Shapey, J, Xia, W, Ourselin, S, Parkin, IP, Desjardins, AE & Vercauteren, T 2019, Soft optically-tuneable fluorescence phantoms based on gel wax and quantum dots: a tissue surrogate for fluorescence imaging validation. Proceedings of SPIE, vol. 10862. https://doi.org/10.1117/12.2508348

APA

Xie, Y., Maneas, E., Islam, S., Peveler, W., Shapey, J., Xia, W., Ourselin, S., Parkin, I. P., Desjardins, A. E., & Vercauteren, T. (2019). Soft optically-tuneable fluorescence phantoms based on gel wax and quantum dots: a tissue surrogate for fluorescence imaging validation. (Proceedings of SPIE). https://doi.org/10.1117/12.2508348

Vancouver

Xie Y, Maneas E, Islam S, Peveler W, Shapey J, Xia W et al. Soft optically-tuneable fluorescence phantoms based on gel wax and quantum dots: a tissue surrogate for fluorescence imaging validation. 2019. (Proceedings of SPIE). https://doi.org/10.1117/12.2508348

Author

Xie, Yijing ; Maneas, Efthymios ; Islam, Shahraz ; Peveler, William ; Shapey, Jonathan ; Xia, Wenfeng ; Ourselin, Sebastien ; Parkin, Ivan P. ; Desjardins, Adrien E. ; Vercauteren, Tom. / Soft optically-tuneable fluorescence phantoms based on gel wax and quantum dots: a tissue surrogate for fluorescence imaging validation. 2019. (Proceedings of SPIE).

Bibtex Download

@book{f7226b1250254bfaae22fba2cbd072c3,
title = "Soft optically-tuneable fluorescence phantoms based on gel wax and quantum dots: a tissue surrogate for fluorescence imaging validation",
abstract = "Fluorescence-guided brain tumour resection, notably using 5-Aminolevulinic acid (ALA)-induced protoporphyrin IX (PpIX) for high-grade gliomas, has been demonstrated to provide better tissue differentiation, thereby improving patient outcomes when compared to white-light guidance. Novel fluorescence imaging devices aiming to increase detection specificity and sensitivity and targeting applications beyond high-grade gliomas are typically assessed by measurements using tissue-mimicking optical phantoms. The field currently lacks adequate phantoms with well-characterised tuneable optical properties. In this study, we developed soft tissue-mimicking fluorescence phantoms (TMFP) highly suitable for this purpose. We investigated: 1) the ability to independently tune optical and fluorescent properties; 2) the stability of the fluorescence signal over time; and 3) the potential of the proposed phantoms for imaging device validation. The TMFP is based on gel-wax which is an optically transparent mineral-oil based soft material. We embedded TiO2 as scattering material, carbon black oil-paint as background absorber, and CdTe Quantum Dots (QDs) as fluorophore because of its similar fluorescence spectrum to PpIX. Scattering and absorption properties were measured by a spectrophotometer, while the fluorescence was assessed by a wide-field fluorescence imaging system (WFFI) and a spectrometer. We demonstrated that: 1) the addition of QDs didn't alter the phantom's scattering which was only defined by the concentration of TiO2, whereas its absorption was defined by both QDs and colour oil paint; 2) the measured fluorescence intensity was linearlyproportional to the concentration of QDs; 3) the fluorescence intensity was stable over time (up to eight months); and 4) the fluorescence signal measured by the WFFI were strongly correlated to spectrometer measurements.",
keywords = "Brain tumour resection, Fluorescence imaging, Gel wax, Quantum dots, Tissue-mimicking phantoms",
author = "Yijing Xie and Efthymios Maneas and Shahraz Islam and William Peveler and Jonathan Shapey and Wenfeng Xia and Sebastien Ourselin and Parkin, {Ivan P.} and Desjardins, {Adrien E.} and Tom Vercauteren",
year = "2019",
doi = "10.1117/12.2508348",
language = "English",
isbn = "978-1-510-62366-8",
volume = "10862",
series = "Proceedings of SPIE",

}

RIS (suitable for import to EndNote) Download

TY - BOOK

T1 - Soft optically-tuneable fluorescence phantoms based on gel wax and quantum dots: a tissue surrogate for fluorescence imaging validation

AU - Xie, Yijing

AU - Maneas, Efthymios

AU - Islam, Shahraz

AU - Peveler, William

AU - Shapey, Jonathan

AU - Xia, Wenfeng

AU - Ourselin, Sebastien

AU - Parkin, Ivan P.

AU - Desjardins, Adrien E.

AU - Vercauteren, Tom

PY - 2019

Y1 - 2019

N2 - Fluorescence-guided brain tumour resection, notably using 5-Aminolevulinic acid (ALA)-induced protoporphyrin IX (PpIX) for high-grade gliomas, has been demonstrated to provide better tissue differentiation, thereby improving patient outcomes when compared to white-light guidance. Novel fluorescence imaging devices aiming to increase detection specificity and sensitivity and targeting applications beyond high-grade gliomas are typically assessed by measurements using tissue-mimicking optical phantoms. The field currently lacks adequate phantoms with well-characterised tuneable optical properties. In this study, we developed soft tissue-mimicking fluorescence phantoms (TMFP) highly suitable for this purpose. We investigated: 1) the ability to independently tune optical and fluorescent properties; 2) the stability of the fluorescence signal over time; and 3) the potential of the proposed phantoms for imaging device validation. The TMFP is based on gel-wax which is an optically transparent mineral-oil based soft material. We embedded TiO2 as scattering material, carbon black oil-paint as background absorber, and CdTe Quantum Dots (QDs) as fluorophore because of its similar fluorescence spectrum to PpIX. Scattering and absorption properties were measured by a spectrophotometer, while the fluorescence was assessed by a wide-field fluorescence imaging system (WFFI) and a spectrometer. We demonstrated that: 1) the addition of QDs didn't alter the phantom's scattering which was only defined by the concentration of TiO2, whereas its absorption was defined by both QDs and colour oil paint; 2) the measured fluorescence intensity was linearlyproportional to the concentration of QDs; 3) the fluorescence intensity was stable over time (up to eight months); and 4) the fluorescence signal measured by the WFFI were strongly correlated to spectrometer measurements.

AB - Fluorescence-guided brain tumour resection, notably using 5-Aminolevulinic acid (ALA)-induced protoporphyrin IX (PpIX) for high-grade gliomas, has been demonstrated to provide better tissue differentiation, thereby improving patient outcomes when compared to white-light guidance. Novel fluorescence imaging devices aiming to increase detection specificity and sensitivity and targeting applications beyond high-grade gliomas are typically assessed by measurements using tissue-mimicking optical phantoms. The field currently lacks adequate phantoms with well-characterised tuneable optical properties. In this study, we developed soft tissue-mimicking fluorescence phantoms (TMFP) highly suitable for this purpose. We investigated: 1) the ability to independently tune optical and fluorescent properties; 2) the stability of the fluorescence signal over time; and 3) the potential of the proposed phantoms for imaging device validation. The TMFP is based on gel-wax which is an optically transparent mineral-oil based soft material. We embedded TiO2 as scattering material, carbon black oil-paint as background absorber, and CdTe Quantum Dots (QDs) as fluorophore because of its similar fluorescence spectrum to PpIX. Scattering and absorption properties were measured by a spectrophotometer, while the fluorescence was assessed by a wide-field fluorescence imaging system (WFFI) and a spectrometer. We demonstrated that: 1) the addition of QDs didn't alter the phantom's scattering which was only defined by the concentration of TiO2, whereas its absorption was defined by both QDs and colour oil paint; 2) the measured fluorescence intensity was linearlyproportional to the concentration of QDs; 3) the fluorescence intensity was stable over time (up to eight months); and 4) the fluorescence signal measured by the WFFI were strongly correlated to spectrometer measurements.

KW - Brain tumour resection

KW - Fluorescence imaging

KW - Gel wax

KW - Quantum dots

KW - Tissue-mimicking phantoms

UR - http://www.scopus.com/inward/record.url?scp=85076812286&partnerID=8YFLogxK

U2 - 10.1117/12.2508348

DO - 10.1117/12.2508348

M3 - Book

SN - 978-1-510-62366-8

VL - 10862

T3 - Proceedings of SPIE

BT - Soft optically-tuneable fluorescence phantoms based on gel wax and quantum dots: a tissue surrogate for fluorescence imaging validation

ER -

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