Abstract
A simplified, automated synthesis for D-[1-C-11]glucose is described by the solid phase-supported reaction of (NH4CN)-C-11 with D-arabinose on an Alumina N Sep-Pak(TM). C-11-cyanide reacted instantaneously to produce [1-C-11]aldonitriles. Reduction with Raney nickel in formic acid and semipreparative HPLC afforded D-[1-C-11]glucose with radiochemical purity >95%. Compared with previously reported procedures a pH adjustment and rotary evaporation step is avoided resulting in a shortening of the synthesis time from 55 to 38 min. The radiochemical yield was 5 - 15% (2.5 - 8.5% non-corrected) based on (NH4CN)-C-11. For a typical production starting with 20 GBq of (NH4CN)-C-11 up to 500 MBq D-[1-C-11]glucose was obtained at end of synthesis as a sterile, formulated solution.
| Original language | English |
|---|---|
| Pages (from-to) | 287-300 |
| Number of pages | 14 |
| Journal | JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS |
| Volume | 41 |
| Issue number | 4 |
| Publication status | Published - Apr 1998 |