Solution NMR assignment of the C-terminal domain of human chTOG

Elena Rostkova; Selena G. Burgess; Richard Bayliss; Mark Pfuhl

doi:10.1007/s12104-018-9812-9

Solution NMR assignment of the C-terminal domain of human chTOG

Elena Rostkova, Selena G. Burgess, Richard Bayliss, Mark Pfuhl^*

^*Corresponding author for this work

Leeds Hosp, University of Leeds

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

The microtubule regulatory protein colonic and hepatic tumor overexpressed gene (chTOG), also known as cytoskeleleton associated protein 5 (CKAP5) plays an important role in organizing the cytoskeleton and in particular in the assembly of k-fibres in mitosis. Recently, we dissected the hitherto poorly understood C-terminus of this protein by discovering two new domains—a cryptic TOG domain (TOG6) and a smaller, helical domain at the very C-terminus. It was shown that the C-terminal domain is important for the interaction with the TACC domain in TACC3 during the assembly of k-fibres in a ternary complex that also includes clathrin. Here we now present the solution NMR assignment of the chTOG C-terminal domain which confirms our earlier prediction that it is mainly made of α-helices. However, the appearance of the ¹H–¹⁵N HSQC spectrum is indicative of the presence of a considerable amount of unstructured and possibly flexible portions of protein in the domain.

Original language	English
Pages (from-to)	1-4
Number of pages	4
Journal	Biomolecular NMR Assignments
Volume	12
Issue number	2
Early online date	26 Mar 2018
DOIs	https://doi.org/10.1007/s12104-018-9812-9
Publication status	E-pub ahead of print - 26 Mar 2018

Keywords

Cell cycle
ChTOG
Kinetochore
Mitosis
TACC3

Access to Document

10.1007/s12104-018-9812-9Licence: CC BY

Cite this

@article{d6e11e01e04c4113a72de0aec8443d74,

title = "Solution NMR assignment of the C-terminal domain of human chTOG",

abstract = "The microtubule regulatory protein colonic and hepatic tumor overexpressed gene (chTOG), also known as cytoskeleleton associated protein 5 (CKAP5) plays an important role in organizing the cytoskeleton and in particular in the assembly of k-fibres in mitosis. Recently, we dissected the hitherto poorly understood C-terminus of this protein by discovering two new domains—a cryptic TOG domain (TOG6) and a smaller, helical domain at the very C-terminus. It was shown that the C-terminal domain is important for the interaction with the TACC domain in TACC3 during the assembly of k-fibres in a ternary complex that also includes clathrin. Here we now present the solution NMR assignment of the chTOG C-terminal domain which confirms our earlier prediction that it is mainly made of α-helices. However, the appearance of the 1H–15N HSQC spectrum is indicative of the presence of a considerable amount of unstructured and possibly flexible portions of protein in the domain.",

keywords = "Cell cycle, ChTOG, Kinetochore, Mitosis, TACC3",

author = "Elena Rostkova and Burgess, {Selena G.} and Richard Bayliss and Mark Pfuhl",

year = "2018",

month = mar,

day = "26",

doi = "10.1007/s12104-018-9812-9",

language = "English",

volume = "12",

pages = "1--4",

journal = "Biomolecular NMR Assignments",

issn = "1874-2718",

publisher = "Springer Netherlands",

number = "2",

}

TY - JOUR

T1 - Solution NMR assignment of the C-terminal domain of human chTOG

AU - Rostkova, Elena

AU - Burgess, Selena G.

AU - Bayliss, Richard

AU - Pfuhl, Mark

PY - 2018/3/26

Y1 - 2018/3/26

N2 - The microtubule regulatory protein colonic and hepatic tumor overexpressed gene (chTOG), also known as cytoskeleleton associated protein 5 (CKAP5) plays an important role in organizing the cytoskeleton and in particular in the assembly of k-fibres in mitosis. Recently, we dissected the hitherto poorly understood C-terminus of this protein by discovering two new domains—a cryptic TOG domain (TOG6) and a smaller, helical domain at the very C-terminus. It was shown that the C-terminal domain is important for the interaction with the TACC domain in TACC3 during the assembly of k-fibres in a ternary complex that also includes clathrin. Here we now present the solution NMR assignment of the chTOG C-terminal domain which confirms our earlier prediction that it is mainly made of α-helices. However, the appearance of the 1H–15N HSQC spectrum is indicative of the presence of a considerable amount of unstructured and possibly flexible portions of protein in the domain.

AB - The microtubule regulatory protein colonic and hepatic tumor overexpressed gene (chTOG), also known as cytoskeleleton associated protein 5 (CKAP5) plays an important role in organizing the cytoskeleton and in particular in the assembly of k-fibres in mitosis. Recently, we dissected the hitherto poorly understood C-terminus of this protein by discovering two new domains—a cryptic TOG domain (TOG6) and a smaller, helical domain at the very C-terminus. It was shown that the C-terminal domain is important for the interaction with the TACC domain in TACC3 during the assembly of k-fibres in a ternary complex that also includes clathrin. Here we now present the solution NMR assignment of the chTOG C-terminal domain which confirms our earlier prediction that it is mainly made of α-helices. However, the appearance of the 1H–15N HSQC spectrum is indicative of the presence of a considerable amount of unstructured and possibly flexible portions of protein in the domain.

KW - Cell cycle

KW - ChTOG

KW - Kinetochore

KW - Mitosis

KW - TACC3

UR - http://www.scopus.com/inward/record.url?scp=85044446046&partnerID=8YFLogxK

U2 - 10.1007/s12104-018-9812-9

DO - 10.1007/s12104-018-9812-9

M3 - Article

AN - SCOPUS:85044446046

SN - 1874-2718

VL - 12

SP - 1

EP - 4

JO - Biomolecular NMR Assignments

JF - Biomolecular NMR Assignments

IS - 2

ER -

Solution NMR assignment of the C-terminal domain of human chTOG

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this