Solution NMR assignment of the C-terminal domain of human chTOG

Elena Rostkova, Selena G. Burgess, Richard Bayliss, Mark Pfuhl*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


The microtubule regulatory protein colonic and hepatic tumor overexpressed gene (chTOG), also known as cytoskeleleton associated protein 5 (CKAP5) plays an important role in organizing the cytoskeleton and in particular in the assembly of k-fibres in mitosis. Recently, we dissected the hitherto poorly understood C-terminus of this protein by discovering two new domains—a cryptic TOG domain (TOG6) and a smaller, helical domain at the very C-terminus. It was shown that the C-terminal domain is important for the interaction with the TACC domain in TACC3 during the assembly of k-fibres in a ternary complex that also includes clathrin. Here we now present the solution NMR assignment of the chTOG C-terminal domain which confirms our earlier prediction that it is mainly made of α-helices. However, the appearance of the 1H–15N HSQC spectrum is indicative of the presence of a considerable amount of unstructured and possibly flexible portions of protein in the domain.

Original languageEnglish
Pages (from-to)1-4
Number of pages4
JournalBiomolecular NMR Assignments
Issue number2
Early online date26 Mar 2018
Publication statusE-pub ahead of print - 26 Mar 2018


  • Cell cycle
  • ChTOG
  • Kinetochore
  • Mitosis
  • TACC3


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