Solution NMR assignment of the cryptic sixth TOG domain of mini spindles

Selena G. Burgess; Richard Bayliss; Mark Pfuhl

doi:10.1007/s12104-015-9620-4

Solution NMR assignment of the cryptic sixth TOG domain of mini spindles

Selena G. Burgess, Richard Bayliss, Mark Pfuhl^*

^*Corresponding author for this work

Institute of Cancer Research

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

TOG domains contribute to the organisation of microtubules through their ability to bind tubulin. They are found in members of the XMAP215 family of proteins, which act as microtubule polymerases and fulfill important roles in the formation of the mitotic spindle and in the assembly of kinetochore fibres. We recently identified a cryptic TOG domain in the XMAP215 family proteins, chTOG and its Drosophila homologue, mini spindles. This domain is not part of the well-established array of TOG domains involved in tubulin polymerisation. Instead it forms part of a binding site for TACC3 family proteins. This interaction is required for the assembly of kinetochore bridges in a trimeric complex with clathrin. Here we present the first NMR assignment of a sixth TOG domain from mini spindles as a first step to elucidate its structure and function.

Original language	English
Pages (from-to)	411-413
Number of pages	3
Journal	Biomolecular NMR Assignments
Volume	9
Issue number	2
DOIs	https://doi.org/10.1007/s12104-015-9620-4
Publication status	Published - 14 May 2015

Keywords

chTOG
HEAT repeats
Kinetochore
Mitosis
TACC3

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10.1007/s12104-015-9620-4

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abstract = "TOG domains contribute to the organisation of microtubules through their ability to bind tubulin. They are found in members of the XMAP215 family of proteins, which act as microtubule polymerases and fulfill important roles in the formation of the mitotic spindle and in the assembly of kinetochore fibres. We recently identified a cryptic TOG domain in the XMAP215 family proteins, chTOG and its Drosophila homologue, mini spindles. This domain is not part of the well-established array of TOG domains involved in tubulin polymerisation. Instead it forms part of a binding site for TACC3 family proteins. This interaction is required for the assembly of kinetochore bridges in a trimeric complex with clathrin. Here we present the first NMR assignment of a sixth TOG domain from mini spindles as a first step to elucidate its structure and function.",

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AU - Burgess, Selena G.

AU - Bayliss, Richard

AU - Pfuhl, Mark

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Solution NMR assignment of the cryptic sixth TOG domain of mini spindles

Abstract

Keywords

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