Solvent-Free Click-Mechanochemistry for the Preparation of Cancer Cell Targeting Graphene Oxide

Noelia Rubio Carrero; Kuo-Ching Mei; Rebecca Klippstein Martin; Pedro Miguel Coutinho Da Costa; Naomi Hodgins; Julie Tzu-Wen Wang; Frederic Festy; Vincenzo Abbate; Robert C Hider; Ka Lung Andrew Chan; Khuloud T Al-Jamal

doi:10.1021/acsami.5b06250

Solvent-Free Click-Mechanochemistry for the Preparation of Cancer Cell Targeting Graphene Oxide

Noelia Rubio Carrero, Kuo-Ching Mei, Rebecca Klippstein Martin, Pedro Miguel Coutinho Da Costa, Naomi Hodgins, Julie Tzu-Wen Wang, Frederic Festy, Vincenzo Abbate, Robert C Hider, Ka Lung Andrew Chan, Khuloud T Al-Jamal

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Abstract

Polyethylene glycol-functionalized nanographene oxide (PEGylated n-GO) was synthesized from alkyne-modified n-GO, using solvent-free click-mechanochemistry, i.e., copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC). The modified n-GO was subsequently conjugated to a mucin 1 receptor immunoglobulin G antibody (anti-MUC1 IgG) via thiol-ene coupling reaction. n-GO derivatives were characterized with Fourier-transformed infrared (FT-IR) spectroscopy, thermogravimetric analysis (TGA), Bradford assay, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and atomic force microscopy (AFM). Cell targeting was confirmed in vitro in MDA-MB-231 cells, either expressing or lacking MUC1 receptors, using flow cytometry, confocal laser scanning microscopy (CLSM) and multiphoton (MP) fluorescence microscopy. Biocompatibility was assessed using the modified lactate dehydrongenase (mLDH) assay.

Original language	English
Pages (from-to)	18920–18923
Journal	ACS Applied Materials and Interfaces
Volume	7
Issue number	34
Early online date	17 Aug 2015
DOIs	https://doi.org/10.1021/acsami.5b06250
Publication status	E-pub ahead of print - 17 Aug 2015

Keywords

antibody; nanomedicine; drug delivery; toxicity; breast cancer

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1021/acsami.5b06250

Rubio et alFinal published version, 1.75 MBLicence: Other

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Rubio Carrero, N., Mei, K.-C., Klippstein Martin, R., Coutinho Da Costa, P. M., Hodgins, N., Wang, J. T.-W., Festy, F., Abbate, V., Hider, R. C., Chan, K. L. A., & Al-Jamal, K. T. (2015). Solvent-Free Click-Mechanochemistry for the Preparation of Cancer Cell Targeting Graphene Oxide. ACS Applied Materials and Interfaces, 7(34), 18920–18923. Advance online publication. https://doi.org/10.1021/acsami.5b06250

@article{9970a9e49b8e40c8840b723683d5d9cd,

title = "Solvent-Free Click-Mechanochemistry for the Preparation of Cancer Cell Targeting Graphene Oxide",

abstract = "Polyethylene glycol-functionalized nanographene oxide (PEGylated n-GO) was synthesized from alkyne-modified n-GO, using solvent-free click-mechanochemistry, i.e., copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC). The modified n-GO was subsequently conjugated to a mucin 1 receptor immunoglobulin G antibody (anti-MUC1 IgG) via thiol-ene coupling reaction. n-GO derivatives were characterized with Fourier-transformed infrared (FT-IR) spectroscopy, thermogravimetric analysis (TGA), Bradford assay, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and atomic force microscopy (AFM). Cell targeting was confirmed in vitro in MDA-MB-231 cells, either expressing or lacking MUC1 receptors, using flow cytometry, confocal laser scanning microscopy (CLSM) and multiphoton (MP) fluorescence microscopy. Biocompatibility was assessed using the modified lactate dehydrongenase (mLDH) assay.",

keywords = "antibody; nanomedicine; drug delivery; toxicity; breast cancer",

author = "{Rubio Carrero}, Noelia and Kuo-Ching Mei and {Klippstein Martin}, Rebecca and {Coutinho Da Costa}, {Pedro Miguel} and Naomi Hodgins and Wang, {Julie Tzu-Wen} and Frederic Festy and Vincenzo Abbate and Hider, {Robert C} and Chan, {Ka Lung Andrew} and Al-Jamal, {Khuloud T}",

year = "2015",

month = aug,

day = "17",

doi = "10.1021/acsami.5b06250",

language = "English",

volume = "7",

pages = "18920–18923",

journal = "ACS Applied Materials and Interfaces",

issn = "1944-8244",

publisher = "American Chemical Society",

number = "34",

}

Rubio Carrero, N , Mei, K-C , Klippstein Martin, R , Coutinho Da Costa, PM , Hodgins, N , Wang, JT-W, Festy, F, Abbate, V , Hider, RC , Chan, KLA & Al-Jamal, KT 2015, 'Solvent-Free Click-Mechanochemistry for the Preparation of Cancer Cell Targeting Graphene Oxide', ACS Applied Materials and Interfaces, vol. 7, no. 34, pp. 18920–18923. https://doi.org/10.1021/acsami.5b06250

TY - JOUR

T1 - Solvent-Free Click-Mechanochemistry for the Preparation of Cancer Cell Targeting Graphene Oxide

AU - Rubio Carrero, Noelia

AU - Mei, Kuo-Ching

AU - Klippstein Martin, Rebecca

AU - Coutinho Da Costa, Pedro Miguel

AU - Hodgins, Naomi

AU - Wang, Julie Tzu-Wen

AU - Festy, Frederic

AU - Abbate, Vincenzo

AU - Hider, Robert C

AU - Chan, Ka Lung Andrew

AU - Al-Jamal, Khuloud T

PY - 2015/8/17

Y1 - 2015/8/17

N2 - Polyethylene glycol-functionalized nanographene oxide (PEGylated n-GO) was synthesized from alkyne-modified n-GO, using solvent-free click-mechanochemistry, i.e., copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC). The modified n-GO was subsequently conjugated to a mucin 1 receptor immunoglobulin G antibody (anti-MUC1 IgG) via thiol-ene coupling reaction. n-GO derivatives were characterized with Fourier-transformed infrared (FT-IR) spectroscopy, thermogravimetric analysis (TGA), Bradford assay, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and atomic force microscopy (AFM). Cell targeting was confirmed in vitro in MDA-MB-231 cells, either expressing or lacking MUC1 receptors, using flow cytometry, confocal laser scanning microscopy (CLSM) and multiphoton (MP) fluorescence microscopy. Biocompatibility was assessed using the modified lactate dehydrongenase (mLDH) assay.

AB - Polyethylene glycol-functionalized nanographene oxide (PEGylated n-GO) was synthesized from alkyne-modified n-GO, using solvent-free click-mechanochemistry, i.e., copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC). The modified n-GO was subsequently conjugated to a mucin 1 receptor immunoglobulin G antibody (anti-MUC1 IgG) via thiol-ene coupling reaction. n-GO derivatives were characterized with Fourier-transformed infrared (FT-IR) spectroscopy, thermogravimetric analysis (TGA), Bradford assay, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and atomic force microscopy (AFM). Cell targeting was confirmed in vitro in MDA-MB-231 cells, either expressing or lacking MUC1 receptors, using flow cytometry, confocal laser scanning microscopy (CLSM) and multiphoton (MP) fluorescence microscopy. Biocompatibility was assessed using the modified lactate dehydrongenase (mLDH) assay.

KW - antibody; nanomedicine; drug delivery; toxicity; breast cancer

U2 - 10.1021/acsami.5b06250

DO - 10.1021/acsami.5b06250

M3 - Letter

C2 - 26278410

SN - 1944-8244

VL - 7

SP - 18920

EP - 18923

JO - ACS Applied Materials and Interfaces

JF - ACS Applied Materials and Interfaces

IS - 34

ER -

Solvent-Free Click-Mechanochemistry for the Preparation of Cancer Cell Targeting Graphene Oxide

Abstract

Keywords

UN SDGs

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