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Solvent-Free Click-Mechanochemistry for the Preparation of Cancer Cell Targeting Graphene Oxide

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Solvent-Free Click-Mechanochemistry for the Preparation of Cancer Cell Targeting Graphene Oxide. / Rubio Carrero, Noelia; Mei, Kuo-Ching; Klippstein Martin, Rebecca; Coutinho Da Costa, Pedro Miguel; Hodgins, Naomi; Wang, Julie Tzu-Wen; Festy, Frederic; Abbate, Vincenzo; Hider, Robert C; Chan, Ka Lung Andrew; Al-Jamal, Khuloud T.

In: ACS Applied Materials and Interfaces, Vol. 7, No. 34, 17.08.2015, p. 18920–18923.

Research output: Contribution to journalLetter

Harvard

Rubio Carrero, N, Mei, K-C, Klippstein Martin, R, Coutinho Da Costa, PM, Hodgins, N, Wang, JT-W, Festy, F, Abbate, V, Hider, RC, Chan, KLA & Al-Jamal, KT 2015, 'Solvent-Free Click-Mechanochemistry for the Preparation of Cancer Cell Targeting Graphene Oxide', ACS Applied Materials and Interfaces, vol. 7, no. 34, pp. 18920–18923. https://doi.org/10.1021/acsami.5b06250

APA

Rubio Carrero, N., Mei, K-C., Klippstein Martin, R., Coutinho Da Costa, P. M., Hodgins, N., Wang, J. T-W., ... Al-Jamal, K. T. (2015). Solvent-Free Click-Mechanochemistry for the Preparation of Cancer Cell Targeting Graphene Oxide. ACS Applied Materials and Interfaces, 7(34), 18920–18923. https://doi.org/10.1021/acsami.5b06250

Vancouver

Rubio Carrero N, Mei K-C, Klippstein Martin R, Coutinho Da Costa PM, Hodgins N, Wang JT-W et al. Solvent-Free Click-Mechanochemistry for the Preparation of Cancer Cell Targeting Graphene Oxide. ACS Applied Materials and Interfaces. 2015 Aug 17;7(34):18920–18923. https://doi.org/10.1021/acsami.5b06250

Author

Rubio Carrero, Noelia ; Mei, Kuo-Ching ; Klippstein Martin, Rebecca ; Coutinho Da Costa, Pedro Miguel ; Hodgins, Naomi ; Wang, Julie Tzu-Wen ; Festy, Frederic ; Abbate, Vincenzo ; Hider, Robert C ; Chan, Ka Lung Andrew ; Al-Jamal, Khuloud T. / Solvent-Free Click-Mechanochemistry for the Preparation of Cancer Cell Targeting Graphene Oxide. In: ACS Applied Materials and Interfaces. 2015 ; Vol. 7, No. 34. pp. 18920–18923.

Bibtex Download

@article{9970a9e49b8e40c8840b723683d5d9cd,
title = "Solvent-Free Click-Mechanochemistry for the Preparation of Cancer Cell Targeting Graphene Oxide",
abstract = "Polyethylene glycol-functionalized nanographene oxide (PEGylated n-GO) was synthesized from alkyne-modified n-GO, using solvent-free click-mechanochemistry, i.e., copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC). The modified n-GO was subsequently conjugated to a mucin 1 receptor immunoglobulin G antibody (anti-MUC1 IgG) via thiol-ene coupling reaction. n-GO derivatives were characterized with Fourier-transformed infrared (FT-IR) spectroscopy, thermogravimetric analysis (TGA), Bradford assay, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and atomic force microscopy (AFM). Cell targeting was confirmed in vitro in MDA-MB-231 cells, either expressing or lacking MUC1 receptors, using flow cytometry, confocal laser scanning microscopy (CLSM) and multiphoton (MP) fluorescence microscopy. Biocompatibility was assessed using the modified lactate dehydrongenase (mLDH) assay.",
keywords = "antibody; nanomedicine; drug delivery; toxicity; breast cancer",
author = "{Rubio Carrero}, Noelia and Kuo-Ching Mei and {Klippstein Martin}, Rebecca and {Coutinho Da Costa}, {Pedro Miguel} and Naomi Hodgins and Wang, {Julie Tzu-Wen} and Frederic Festy and Vincenzo Abbate and Hider, {Robert C} and Chan, {Ka Lung Andrew} and Al-Jamal, {Khuloud T}",
year = "2015",
month = "8",
day = "17",
doi = "10.1021/acsami.5b06250",
language = "English",
volume = "7",
pages = "18920–18923",
journal = "ACS Applied Materials and Interfaces",
issn = "1944-8244",
publisher = "American Chemical Society",
number = "34",

}

RIS (suitable for import to EndNote) Download

TY - JOUR

T1 - Solvent-Free Click-Mechanochemistry for the Preparation of Cancer Cell Targeting Graphene Oxide

AU - Rubio Carrero, Noelia

AU - Mei, Kuo-Ching

AU - Klippstein Martin, Rebecca

AU - Coutinho Da Costa, Pedro Miguel

AU - Hodgins, Naomi

AU - Wang, Julie Tzu-Wen

AU - Festy, Frederic

AU - Abbate, Vincenzo

AU - Hider, Robert C

AU - Chan, Ka Lung Andrew

AU - Al-Jamal, Khuloud T

PY - 2015/8/17

Y1 - 2015/8/17

N2 - Polyethylene glycol-functionalized nanographene oxide (PEGylated n-GO) was synthesized from alkyne-modified n-GO, using solvent-free click-mechanochemistry, i.e., copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC). The modified n-GO was subsequently conjugated to a mucin 1 receptor immunoglobulin G antibody (anti-MUC1 IgG) via thiol-ene coupling reaction. n-GO derivatives were characterized with Fourier-transformed infrared (FT-IR) spectroscopy, thermogravimetric analysis (TGA), Bradford assay, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and atomic force microscopy (AFM). Cell targeting was confirmed in vitro in MDA-MB-231 cells, either expressing or lacking MUC1 receptors, using flow cytometry, confocal laser scanning microscopy (CLSM) and multiphoton (MP) fluorescence microscopy. Biocompatibility was assessed using the modified lactate dehydrongenase (mLDH) assay.

AB - Polyethylene glycol-functionalized nanographene oxide (PEGylated n-GO) was synthesized from alkyne-modified n-GO, using solvent-free click-mechanochemistry, i.e., copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC). The modified n-GO was subsequently conjugated to a mucin 1 receptor immunoglobulin G antibody (anti-MUC1 IgG) via thiol-ene coupling reaction. n-GO derivatives were characterized with Fourier-transformed infrared (FT-IR) spectroscopy, thermogravimetric analysis (TGA), Bradford assay, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and atomic force microscopy (AFM). Cell targeting was confirmed in vitro in MDA-MB-231 cells, either expressing or lacking MUC1 receptors, using flow cytometry, confocal laser scanning microscopy (CLSM) and multiphoton (MP) fluorescence microscopy. Biocompatibility was assessed using the modified lactate dehydrongenase (mLDH) assay.

KW - antibody; nanomedicine; drug delivery; toxicity; breast cancer

U2 - 10.1021/acsami.5b06250

DO - 10.1021/acsami.5b06250

M3 - Letter

C2 - 26278410

VL - 7

SP - 18920

EP - 18923

JO - ACS Applied Materials and Interfaces

JF - ACS Applied Materials and Interfaces

SN - 1944-8244

IS - 34

ER -

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