SORL1 and SIRT1 mRNA expression and promoter methylation levels in aging and Alzheimer's Disease

Tatiane Katsue Furuya; Patricia Natalia Oliveira da Silva; Spencer Luiz Marques Payao; Lucas Trevizani Rasmussen; Roger Willian de Labio; Paulo Henrique Ferreira Bertolucci; Ianna Lacerda Sampaio Braga; Elizabeth Suchi Chen; Gustavo Turecki; Naguib Mechawar; Jonathan Mill; Marilia de Arruda Cardoso Smith

doi:10.1016/j.neuint.2012.07.014

SORL1 and SIRT1 mRNA expression and promoter methylation levels in aging and Alzheimer's Disease

Tatiane Katsue Furuya, Patricia Natalia Oliveira da Silva, Spencer Luiz Marques Payao, Lucas Trevizani Rasmussen, Roger Willian de Labio, Paulo Henrique Ferreira Bertolucci, Ianna Lacerda Sampaio Braga, Elizabeth Suchi Chen, Gustavo Turecki, Naguib Mechawar, Jonathan Mill, Marilia de Arruda Cardoso Smith^*

^*Corresponding author for this work

Social Genetic & Developmental Psychiatry

Research output: Contribution to journal › Article › peer-review

57 Citations (Scopus)

Abstract

Alzheimer's Disease (AD) is a neurodegenerative disorder and the most common cause of dementia among the elderly. Efforts have been made to understand the genetic and epigenetic mechanisms involved in the development of this disease. As SORL1 (sortilin-related receptor) and SIRT1 (sirtuin I) genes have been linked to AD pathogenesis, we aimed to investigate their mRNA expression and promoter DNA methylation in post mortem brain tissues (entorhinal and auditory cortices and hippocampus) from healthy elderly subjects and AD patients. We also evaluated these levels in peripheral blood leukocytes from young, healthy elderly and AD patients, investigating whether there was an effect of age on these profiles. The comparative CT method by Real Time PCR and MALDI-TOF mass spectrometry were used to analyze gene expression and DNA methylation, respectively. SORL1 gene was differently expressed in the peripheral blood leukocytes and might act as a marker of aging in this tissue Furthermore, we found that SORL1 promoter DNA methylation might act as one of the mechanisms responsible for the differences in expression observed between blood and brain for both healthy elderly and AD patients groups. The impact of these studied genes on AD pathogenesis remains to be better clarified.

Original language	English
Article number	N/A
Pages (from-to)	973-975
Number of pages	3
Journal	Neurochemistry International
Volume	61
Issue number	7
DOIs	https://doi.org/10.1016/j.neuint.2012.07.014
Publication status	Published - Dec 2012

Keywords

Alzheimer's Disease
Aging
SORL1 and SIRT1 mRNA expression
Peripheral blood leukocytes
Post mortem brain tissue
Promoter DNA methylation
RECEPTOR LR11

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10.1016/j.neuint.2012.07.014

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Furuya, T. K., Oliveira da Silva, P. N., Marques Payao, S. L., Rasmussen, L. T., de Labio, R. W., Ferreira Bertolucci, P. H., Sampaio Braga, I. L., Chen, E. S., Turecki, G., Mechawar, N., Mill, J., & Cardoso Smith, M. D. A. (2012). SORL1 and SIRT1 mRNA expression and promoter methylation levels in aging and Alzheimer's Disease. Neurochemistry International, 61(7), 973-975. Article N/A. https://doi.org/10.1016/j.neuint.2012.07.014

@article{13c484f2cc9a4ccbae7d54cddb3f19b1,

title = "SORL1 and SIRT1 mRNA expression and promoter methylation levels in aging and Alzheimer's Disease",

abstract = "Alzheimer's Disease (AD) is a neurodegenerative disorder and the most common cause of dementia among the elderly. Efforts have been made to understand the genetic and epigenetic mechanisms involved in the development of this disease. As SORL1 (sortilin-related receptor) and SIRT1 (sirtuin I) genes have been linked to AD pathogenesis, we aimed to investigate their mRNA expression and promoter DNA methylation in post mortem brain tissues (entorhinal and auditory cortices and hippocampus) from healthy elderly subjects and AD patients. We also evaluated these levels in peripheral blood leukocytes from young, healthy elderly and AD patients, investigating whether there was an effect of age on these profiles. The comparative CT method by Real Time PCR and MALDI-TOF mass spectrometry were used to analyze gene expression and DNA methylation, respectively. SORL1 gene was differently expressed in the peripheral blood leukocytes and might act as a marker of aging in this tissue Furthermore, we found that SORL1 promoter DNA methylation might act as one of the mechanisms responsible for the differences in expression observed between blood and brain for both healthy elderly and AD patients groups. The impact of these studied genes on AD pathogenesis remains to be better clarified. ",

keywords = "Alzheimer's Disease, Aging, SORL1 and SIRT1 mRNA expression, Peripheral blood leukocytes, Post mortem brain tissue, Promoter DNA methylation, RECEPTOR LR11",

author = "Furuya, {Tatiane Katsue} and {Oliveira da Silva}, {Patricia Natalia} and {Marques Payao}, {Spencer Luiz} and Rasmussen, {Lucas Trevizani} and {de Labio}, {Roger Willian} and {Ferreira Bertolucci}, {Paulo Henrique} and {Sampaio Braga}, {Ianna Lacerda} and Chen, {Elizabeth Suchi} and Gustavo Turecki and Naguib Mechawar and Jonathan Mill and {Cardoso Smith}, {Marilia de Arruda}",

year = "2012",

month = dec,

doi = "10.1016/j.neuint.2012.07.014",

language = "English",

volume = "61",

pages = "973--975",

journal = "Neurochemistry International",

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Furuya, TK, Oliveira da Silva, PN, Marques Payao, SL, Rasmussen, LT, de Labio, RW, Ferreira Bertolucci, PH, Sampaio Braga, IL, Chen, ES, Turecki, G, Mechawar, N, Mill, J & Cardoso Smith, MDA 2012, 'SORL1 and SIRT1 mRNA expression and promoter methylation levels in aging and Alzheimer's Disease', Neurochemistry International, vol. 61, no. 7, N/A, pp. 973-975. https://doi.org/10.1016/j.neuint.2012.07.014

TY - JOUR

T1 - SORL1 and SIRT1 mRNA expression and promoter methylation levels in aging and Alzheimer's Disease

AU - Furuya, Tatiane Katsue

AU - Oliveira da Silva, Patricia Natalia

AU - Marques Payao, Spencer Luiz

AU - Rasmussen, Lucas Trevizani

AU - de Labio, Roger Willian

AU - Ferreira Bertolucci, Paulo Henrique

AU - Sampaio Braga, Ianna Lacerda

AU - Chen, Elizabeth Suchi

AU - Turecki, Gustavo

AU - Mechawar, Naguib

AU - Mill, Jonathan

AU - Cardoso Smith, Marilia de Arruda

PY - 2012/12

Y1 - 2012/12

N2 - Alzheimer's Disease (AD) is a neurodegenerative disorder and the most common cause of dementia among the elderly. Efforts have been made to understand the genetic and epigenetic mechanisms involved in the development of this disease. As SORL1 (sortilin-related receptor) and SIRT1 (sirtuin I) genes have been linked to AD pathogenesis, we aimed to investigate their mRNA expression and promoter DNA methylation in post mortem brain tissues (entorhinal and auditory cortices and hippocampus) from healthy elderly subjects and AD patients. We also evaluated these levels in peripheral blood leukocytes from young, healthy elderly and AD patients, investigating whether there was an effect of age on these profiles. The comparative CT method by Real Time PCR and MALDI-TOF mass spectrometry were used to analyze gene expression and DNA methylation, respectively. SORL1 gene was differently expressed in the peripheral blood leukocytes and might act as a marker of aging in this tissue Furthermore, we found that SORL1 promoter DNA methylation might act as one of the mechanisms responsible for the differences in expression observed between blood and brain for both healthy elderly and AD patients groups. The impact of these studied genes on AD pathogenesis remains to be better clarified.

AB - Alzheimer's Disease (AD) is a neurodegenerative disorder and the most common cause of dementia among the elderly. Efforts have been made to understand the genetic and epigenetic mechanisms involved in the development of this disease. As SORL1 (sortilin-related receptor) and SIRT1 (sirtuin I) genes have been linked to AD pathogenesis, we aimed to investigate their mRNA expression and promoter DNA methylation in post mortem brain tissues (entorhinal and auditory cortices and hippocampus) from healthy elderly subjects and AD patients. We also evaluated these levels in peripheral blood leukocytes from young, healthy elderly and AD patients, investigating whether there was an effect of age on these profiles. The comparative CT method by Real Time PCR and MALDI-TOF mass spectrometry were used to analyze gene expression and DNA methylation, respectively. SORL1 gene was differently expressed in the peripheral blood leukocytes and might act as a marker of aging in this tissue Furthermore, we found that SORL1 promoter DNA methylation might act as one of the mechanisms responsible for the differences in expression observed between blood and brain for both healthy elderly and AD patients groups. The impact of these studied genes on AD pathogenesis remains to be better clarified.

KW - Alzheimer's Disease

KW - Aging

KW - SORL1 and SIRT1 mRNA expression

KW - Peripheral blood leukocytes

KW - Post mortem brain tissue

KW - Promoter DNA methylation

KW - RECEPTOR LR11

U2 - 10.1016/j.neuint.2012.07.014

DO - 10.1016/j.neuint.2012.07.014

M3 - Article

SN - 0197-0186

VL - 61

SP - 973

EP - 975

JO - Neurochemistry International

JF - Neurochemistry International

IS - 7

M1 - N/A

ER -

SORL1 and SIRT1 mRNA expression and promoter methylation levels in aging and Alzheimer's Disease

Abstract

Keywords

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