Sparse feature selection methods identify unexpected global cellular response to strontium-containing materials

Helene Autefage, Eileen Gentleman, Elena Littmann, Martin A. B. Hedegaard, Thomas Von Erlach, Matthew O'Donnell, Frank R. Burden, David A. Winkler, Molly M. Stevens*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

59 Citations (Scopus)
205 Downloads (Pure)

Abstract

Despite the increasing sophistication of biomaterials design and functional characterization studies, little is known regarding cells' global response to biomaterials. Here, we combined nontargeted holistic biological and physical science techniques to evaluate how simple strontium ion incorporation within the well-described biomaterial 45S5 bioactive glass (BG) influences the global response of human mesenchymal stem cells. Our objective analyses of whole gene-expression profiles, confirmed by standard molecular biology techniques, revealed that strontium-substituted BG up-regulated the isoprenoid pathway, suggesting an influence on both sterol metabolite synthesis and protein prenylation processes. This up-regulation was accompanied by increases in cellular and membrane cholesterol and lipid raft contents as determined by Raman spectroscopy mapping and total internal reflection fluorescence microscopy analyses and by an increase in cellular content of phosphorylated myosin II light chain. Our unexpected findings of this strong metabolic pathway regulation as a response to biomaterial composition highlight the benefits of discovery-driven nonreductionist approaches to gain a deeper understanding of global cell-material interactions and suggest alternative research routes for evaluating biomaterials to improve their design.

Original languageEnglish
Pages (from-to)4280-4285
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume112
Issue number14
DOIs
Publication statusPublished - 7 Apr 2015

Keywords

  • strontium-releasing biomaterials
  • human mesenchymal stem cells
  • microarray analysis
  • sparse feature selection analysis
  • mevalonate pathway
  • MESENCHYMAL STROMAL CELLS
  • OSTEOGENIC DIFFERENTIATION
  • OSTEOBLAST DIFFERENTIATION
  • MOLECULAR-MECHANISMS
  • DESCRIPTOR SELECTION
  • SQUALENE SYNTHASE
  • LIPID RAFTS
  • RANELATE
  • OSTEOPOROSIS
  • CHOLESTEROL

Fingerprint

Dive into the research topics of 'Sparse feature selection methods identify unexpected global cellular response to strontium-containing materials'. Together they form a unique fingerprint.

Cite this