@article{2b8950133f4545d88f54e39590877c1e,
title = "Splicing factors Sf3A2 and Prp31 have direct roles in mitotic chromosome segregation",
abstract = "Several studies have shown that RNAi-mediated depletion of splicing factors (SFs) results in mitotic abnormalities. However, it is currently unclear whether these abnormalities reflect defective splicing of specific pre-mRNAs or a direct role of the SFs in mitosis. Here, we show that two highly conserved SFs, Sf3A2 and Prp31, are required for chromosome segregation in both Drosophila and human cells. Injections of anti-Sf3A2 and anti-Prp31 antibodies into Drosophila embryos disrupt mitotic division within 1 min, arguing strongly against a splicing-related mitotic function of these factors. We demonstrate that both SFs bind spindle microtubules (MTs) and the Ndc80 complex, which in Sf3A2-and Prp31-depleted cells is not tightly associated with the kinetochores; in HeLa cells the Ndc80/HEC1-SF interaction is restricted to the M phase. These results indicate that Sf3A2 and Prp31 directly regulate interactions among kinetochores, spindle microtubules and the Ndc80 complex in both Drosophila and human cells.",
author = "Claudia Pellacani and Elisabetta Bucciarelli and Fioranna Renda and Daniel Hayward and Antonella Palena and Jack Chen and Silvia Bonaccorsi and Wakefield, {James G.} and Maurizio Gatti and Somma, {Maria Patrizia}",
note = "Funding Information: We thank David Glover, Mike Goldberg, Gohta Goshima, Gary Karpen, Christian Lehner and Stefano Sechi for their generous gifts of antibodies and cell lines, and Giuseppe Bosso for the GST-tagged telomere proteins. We thank Kate Heesom, of the Bristol Proteomics Facility, for processing the TMT quantitative proteomics samples, and Ryan Ames for assisting in the proteome analysis. The monoclonal anti-lamin Dm0 was obtained from the Hybridoma Bank, created by the NICHD of the NIH and maintained at the University of Iowa, Department of Biology, Iowa City, IA 52242. This work was supported by grants from Italian Association for Cancer Research to MG (AIRC, IG16020 and 20528), a PRIN grant from MIUR to SB, a BBSRC grant (BB/K017837/1) to JGW and a BBSRC PhD studentship to DH. Publisher Copyright: {\textcopyright} Pellacani et al.",
year = "2018",
month = nov,
day = "1",
doi = "10.7554/eLife.40325",
language = "English",
volume = "7",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications, Ltd",
}
