Statistical optimization of enzymatic synthesis of derivatives of trehalose and sucrose

N. K. Smith, S. G. Gilmour, R. A. Rastall*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

The synthesis of the oligosaccharides 6-O-α-mannosyltrehalose, 6-O-α-glucosyltrehalose, 6-O-α-galactosyltrehalose, and 6-O-g-α-mannosylsucrose, using appropriate glucosidases was optimized using response surface methodology. An empirical mathematical model was constructed to predict the amount of product formed under various conditions of pH, substrate concentration, and donor;acceptor ratio.

Original languageEnglish
Pages (from-to)349-354
Number of pages6
JournalENZYME AND MICROBIAL TECHNOLOGY
Volume21
Issue number5
DOIs
Publication statusPublished - Oct 1997

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