Stem Cell-derived neurons from autistic individuals with SHANK3 mutation show morphogenetic abnormalities during early development

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Abstract

Shank3 is a structural protein found predominantly at the post-synaptic density. Mutations in the SHANK3 gene have been associated with risk for autism spectrum disorder (ASD). We generated induced pluripotent stem cells (iPSCs) from control individuals and from human donors with ASD, carrying microdeletions of SHANK3. In addition, we used Zinc finger nucleases to generate isogenic SHANK3 knockout human embryonic stem (ES) cell lines. We differentiated pluripotent cells into either cortical or olfactory placodal neurons. We show that patient-derived placodal neurons make fewer synapses than control cells. Moreover, patient-derived cells display a developmental phenotype: young post-mitotic neurons have smaller cell bodies, more extensively branched neurites, and reduced motility compared with controls. These phenotypes were mimicked by SHANK3-edited ES cells, and rescued by transduction with a Shank3 expression construct. This developmental phenotype is not observed in the same iPSC lines differentiated into cortical neurons. Therefore, we suggest that SHANK3 plays a critical role in neuronal morphogenesis in placodal neurons, and that early defects are associated with ASD-associated mutations.
Original languageEnglish
Pages (from-to)735-746
JournalMolecular Psychiatry
Volume23
Issue number3
Early online date26 Sept 2017
DOIs
Publication statusPublished - 1 Mar 2018

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