Stem cell function, self-renewal and behavioural heterogeneity of cells from the adult muscle satellite cell niche

TY - JOUR

T1 - Stem cell function, self-renewal and behavioural heterogeneity of cells from the adult muscle satellite cell niche

AU - Collins, Charlotte

AU - Olsen, Irwin

AU - Zammit, Peter

AU - Heslop, Louise

AU - Petrie, Aviva

AU - Partridge, Terence

AU - Morgan, Jennifer

PY - 2005/7/29

Y1 - 2005/7/29

N2 - Satellite cells are situated beneath the basal lamina that surrounds each myofiber and function as myogenic precursors for muscle growth and repair. The source of satellite cell renewal is controversial and has been suggested to be a separate circulating or interstitial stem cell population. Here, we transplant single intact myofibers into radiation-ablated muscles and demonstrate that satellite cells are self-sufficient as a source of regeneration. As few as seven satellite cells associated with one transplanted myofiber can generate over 100 new myofibers containing thousands of myonuclei. Moreover, the transplanted satellite cells vigorously self-renew, expanding in number and repopulating the host muscle with new satellite cells. Following experimental injury, these cells proliferate extensively and regenerate large compact clusters of myofibers. Thus, within a normally stable tissue, the satellite cell exhibits archetypal stem cell properties and is competent to form the basal origin of adult muscle regeneration.

AB - Satellite cells are situated beneath the basal lamina that surrounds each myofiber and function as myogenic precursors for muscle growth and repair. The source of satellite cell renewal is controversial and has been suggested to be a separate circulating or interstitial stem cell population. Here, we transplant single intact myofibers into radiation-ablated muscles and demonstrate that satellite cells are self-sufficient as a source of regeneration. As few as seven satellite cells associated with one transplanted myofiber can generate over 100 new myofibers containing thousands of myonuclei. Moreover, the transplanted satellite cells vigorously self-renew, expanding in number and repopulating the host muscle with new satellite cells. Following experimental injury, these cells proliferate extensively and regenerate large compact clusters of myofibers. Thus, within a normally stable tissue, the satellite cell exhibits archetypal stem cell properties and is competent to form the basal origin of adult muscle regeneration.

U2 - 10.1016/j.cell.2005.05.010

DO - 10.1016/j.cell.2005.05.010

M3 - Article

VL - 122

SP - 289

EP - 301

JO - Cell

JF - Cell

IS - 2

ER -