Stem Cell Research Lab Resource: Stem Cell LineInduced pluripotent stem cell (iPSC) line MLi-003A derived from an individual with the maximum number of filaggrin (FLG) tandem repeats

Preeti Khurana; Nikola Kolundzic; Marija Rogar; Carl Hobbs; X. F.Colin C. Wong; John E.A. Common; Dusko Ilic; Mirjana Liovic

doi:10.1016/j.scr.2020.101827

Stem Cell Research Lab Resource: Stem Cell LineInduced pluripotent stem cell (iPSC) line MLi-003A derived from an individual with the maximum number of filaggrin (FLG) tandem repeats

Preeti Khurana, Nikola Kolundzic, Marija Rogar, Carl Hobbs, X. F.Colin C. Wong, John E.A. Common, Dusko Ilic, Mirjana Liovic^*

^*Corresponding author for this work

Women & Children's Health

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

We have generated MLi003-A, a new induced pluripotent stem cell (iPSC) line derived from hair follicle keratinocytes of a healthy male characterized with a maximum number of filaggrin tandem repeats, making this iPSC line the best control for studies on skin barrier function. The characterization of the MLi003-A cell line consisted of molecular karyotyping, high-throughput array-based sequencing composed of Fluidigm microfluidics technology and next-generation sequencing of the filaggrin alleles, and pluripotency and differentiation potentials testing by immunofluorescence of associated markers both in vitro and in vivo. The MLi-003A line has been also tested for ability to differentiate into keratinocytes.

Original language	English
Article number	101827
Journal	Stem Cell Research
Volume	45
DOIs	https://doi.org/10.1016/j.scr.2020.101827
Publication status	Published - May 2020

Keywords

Atopic dermatitis
Filaggrin
IPSC line
Keratinocyte
Pluripotency

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10.1016/j.scr.2020.101827

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Khurana, P., Kolundzic, N., Rogar, M., Hobbs, C., Wong, X. F. C. C., Common, J. E. A., Ilic, D., & Liovic, M. (2020). Stem Cell Research Lab Resource: Stem Cell LineInduced pluripotent stem cell (iPSC) line MLi-003A derived from an individual with the maximum number of filaggrin (FLG) tandem repeats. Stem Cell Research, 45, Article 101827. https://doi.org/10.1016/j.scr.2020.101827

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title = "Stem Cell Research Lab Resource: Stem Cell LineInduced pluripotent stem cell (iPSC) line MLi-003A derived from an individual with the maximum number of filaggrin (FLG) tandem repeats",

abstract = "We have generated MLi003-A, a new induced pluripotent stem cell (iPSC) line derived from hair follicle keratinocytes of a healthy male characterized with a maximum number of filaggrin tandem repeats, making this iPSC line the best control for studies on skin barrier function. The characterization of the MLi003-A cell line consisted of molecular karyotyping, high-throughput array-based sequencing composed of Fluidigm microfluidics technology and next-generation sequencing of the filaggrin alleles, and pluripotency and differentiation potentials testing by immunofluorescence of associated markers both in vitro and in vivo. The MLi-003A line has been also tested for ability to differentiate into keratinocytes.",

keywords = "Atopic dermatitis, Filaggrin, IPSC line, Keratinocyte, Pluripotency",

author = "Preeti Khurana and Nikola Kolundzic and Marija Rogar and Carl Hobbs and Wong, {X. F.Colin C.} and Common, {John E.A.} and Dusko Ilic and Mirjana Liovic",

year = "2020",

month = may,

doi = "10.1016/j.scr.2020.101827",

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AU - Khurana, Preeti

AU - Kolundzic, Nikola

AU - Rogar, Marija

AU - Hobbs, Carl

AU - Wong, X. F.Colin C.

AU - Common, John E.A.

AU - Ilic, Dusko

AU - Liovic, Mirjana

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AB - We have generated MLi003-A, a new induced pluripotent stem cell (iPSC) line derived from hair follicle keratinocytes of a healthy male characterized with a maximum number of filaggrin tandem repeats, making this iPSC line the best control for studies on skin barrier function. The characterization of the MLi003-A cell line consisted of molecular karyotyping, high-throughput array-based sequencing composed of Fluidigm microfluidics technology and next-generation sequencing of the filaggrin alleles, and pluripotency and differentiation potentials testing by immunofluorescence of associated markers both in vitro and in vivo. The MLi-003A line has been also tested for ability to differentiate into keratinocytes.

KW - Atopic dermatitis

KW - Filaggrin

KW - IPSC line

KW - Keratinocyte

KW - Pluripotency

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Stem Cell Research Lab Resource: Stem Cell LineInduced pluripotent stem cell (iPSC) line MLi-003A derived from an individual with the maximum number of filaggrin (FLG) tandem repeats

Abstract

Keywords

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