Stem Cell Research Lab Resource: Stem Cell LineInduced pluripotent stem cell (iPSC) line MLi-003A derived from an individual with the maximum number of filaggrin (FLG) tandem repeats

Preeti Khurana; Nikola Kolundzic; Marija Rogar; Carl Hobbs; X. F.Colin C. Wong; John E.A. Common; Dusko Ilic; Mirjana Liovic

doi:10.1016/j.scr.2020.101827

Stem Cell Research Lab Resource: Stem Cell LineInduced pluripotent stem cell (iPSC) line MLi-003A derived from an individual with the maximum number of filaggrin (FLG) tandem repeats

Preeti Khurana
, Nikola Kolundzic
, Marija Rogar
, Carl Hobbs
, X. F.Colin C. Wong
, John E.A. Common
, Dusko Ilic
, Mirjana Liovic^*

^*Corresponding author for this work

Women & Children's Health

Department of Women and Children's Health
King's College London
University of Ljubljana
AdriaBio doo
A*STAR

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

We have generated MLi003-A, a new induced pluripotent stem cell (iPSC) line derived from hair follicle keratinocytes of a healthy male characterized with a maximum number of filaggrin tandem repeats, making this iPSC line the best control for studies on skin barrier function. The characterization of the MLi003-A cell line consisted of molecular karyotyping, high-throughput array-based sequencing composed of Fluidigm microfluidics technology and next-generation sequencing of the filaggrin alleles, and pluripotency and differentiation potentials testing by immunofluorescence of associated markers both in vitro and in vivo. The MLi-003A line has been also tested for ability to differentiate into keratinocytes.

Original language	English
Article number	101827
Journal	Stem Cell Research
Volume	45
DOIs	https://doi.org/10.1016/j.scr.2020.101827
Publication status	Published - May 2020

Keywords

Atopic dermatitis
Filaggrin
IPSC line
Keratinocyte
Pluripotency

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10.1016/j.scr.2020.101827

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Khurana, P., Kolundzic, N., Rogar, M., Hobbs, C., Wong, X. F. C. C., Common, J. E. A., Ilic, D., & Liovic, M. (2020). Stem Cell Research Lab Resource: Stem Cell LineInduced pluripotent stem cell (iPSC) line MLi-003A derived from an individual with the maximum number of filaggrin (FLG) tandem repeats. Stem Cell Research, 45, Article 101827. https://doi.org/10.1016/j.scr.2020.101827

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title = "Stem Cell Research Lab Resource: Stem Cell LineInduced pluripotent stem cell (iPSC) line MLi-003A derived from an individual with the maximum number of filaggrin (FLG) tandem repeats",

abstract = "We have generated MLi003-A, a new induced pluripotent stem cell (iPSC) line derived from hair follicle keratinocytes of a healthy male characterized with a maximum number of filaggrin tandem repeats, making this iPSC line the best control for studies on skin barrier function. The characterization of the MLi003-A cell line consisted of molecular karyotyping, high-throughput array-based sequencing composed of Fluidigm microfluidics technology and next-generation sequencing of the filaggrin alleles, and pluripotency and differentiation potentials testing by immunofluorescence of associated markers both in vitro and in vivo. The MLi-003A line has been also tested for ability to differentiate into keratinocytes.",

keywords = "Atopic dermatitis, Filaggrin, IPSC line, Keratinocyte, Pluripotency",

author = "Preeti Khurana and Nikola Kolundzic and Marija Rogar and Carl Hobbs and Wong, \{X. F.Colin C.\} and Common, \{John E.A.\} and Dusko Ilic and Mirjana Liovic",

year = "2020",

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doi = "10.1016/j.scr.2020.101827",

language = "English",

volume = "45",

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AU - Khurana, Preeti

AU - Kolundzic, Nikola

AU - Rogar, Marija

AU - Hobbs, Carl

AU - Wong, X. F.Colin C.

AU - Common, John E.A.

AU - Ilic, Dusko

AU - Liovic, Mirjana

PY - 2020/5

Y1 - 2020/5

N2 - We have generated MLi003-A, a new induced pluripotent stem cell (iPSC) line derived from hair follicle keratinocytes of a healthy male characterized with a maximum number of filaggrin tandem repeats, making this iPSC line the best control for studies on skin barrier function. The characterization of the MLi003-A cell line consisted of molecular karyotyping, high-throughput array-based sequencing composed of Fluidigm microfluidics technology and next-generation sequencing of the filaggrin alleles, and pluripotency and differentiation potentials testing by immunofluorescence of associated markers both in vitro and in vivo. The MLi-003A line has been also tested for ability to differentiate into keratinocytes.

AB - We have generated MLi003-A, a new induced pluripotent stem cell (iPSC) line derived from hair follicle keratinocytes of a healthy male characterized with a maximum number of filaggrin tandem repeats, making this iPSC line the best control for studies on skin barrier function. The characterization of the MLi003-A cell line consisted of molecular karyotyping, high-throughput array-based sequencing composed of Fluidigm microfluidics technology and next-generation sequencing of the filaggrin alleles, and pluripotency and differentiation potentials testing by immunofluorescence of associated markers both in vitro and in vivo. The MLi-003A line has been also tested for ability to differentiate into keratinocytes.

KW - Atopic dermatitis

KW - Filaggrin

KW - IPSC line

KW - Keratinocyte

KW - Pluripotency

UR - https://www.scopus.com/pages/publications/85083805891

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DO - 10.1016/j.scr.2020.101827

M3 - Article

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SN - 1873-5061

VL - 45

JO - Stem Cell Research

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ER -

Stem Cell Research Lab Resource: Stem Cell LineInduced pluripotent stem cell (iPSC) line MLi-003A derived from an individual with the maximum number of filaggrin (FLG) tandem repeats

Abstract

Keywords

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