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Stochastic Choice of Allelic Expression in Human Neural Stem Cells

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Stochastic Choice of Allelic Expression in Human Neural Stem Cells. / Jeffries, Aaron R.; Perfect, Leo; Ledderose, Julia; Schalkwyk, Leonard C.; Bray, Nicholas J.; Mill, Jonathan; Price, Jack.

In: Stem Cells, Vol. 30, No. 9, 09.2012, p. 1938-1947.

Research output: Contribution to journalArticle

Harvard

Jeffries, AR, Perfect, L, Ledderose, J, Schalkwyk, LC, Bray, NJ, Mill, J & Price, J 2012, 'Stochastic Choice of Allelic Expression in Human Neural Stem Cells', Stem Cells, vol. 30, no. 9, pp. 1938-1947. https://doi.org/10.1002/stem.1155

APA

Jeffries, A. R., Perfect, L., Ledderose, J., Schalkwyk, L. C., Bray, N. J., Mill, J., & Price, J. (2012). Stochastic Choice of Allelic Expression in Human Neural Stem Cells. Stem Cells, 30(9), 1938-1947. https://doi.org/10.1002/stem.1155

Vancouver

Jeffries AR, Perfect L, Ledderose J, Schalkwyk LC, Bray NJ, Mill J et al. Stochastic Choice of Allelic Expression in Human Neural Stem Cells. Stem Cells. 2012 Sep;30(9):1938-1947. https://doi.org/10.1002/stem.1155

Author

Jeffries, Aaron R. ; Perfect, Leo ; Ledderose, Julia ; Schalkwyk, Leonard C. ; Bray, Nicholas J. ; Mill, Jonathan ; Price, Jack. / Stochastic Choice of Allelic Expression in Human Neural Stem Cells. In: Stem Cells. 2012 ; Vol. 30, No. 9. pp. 1938-1947.

Bibtex Download

@article{43d0eaf641d6471a877f3050b4947d73,
title = "Stochastic Choice of Allelic Expression in Human Neural Stem Cells",
abstract = "Monoallelic gene expression, such as genomic imprinting, is well described. Less well-characterized are genes undergoing stochastic monoallelic expression (MA), where specific clones of cells express just one allele at a given locus. We performed genome-wide allelic expression assessment of human clonal neural stem cells derived from cerebral cortex, striatum, and spinal cord, each with differing genotypes. We assayed three separate clonal lines from each donor, distinguishing stochastic MA from genotypic effects. Roughly 2% of genes showed evidence for autosomal MA, and in about half of these, allelic expression was stochastic between different clones. Many of these loci were known neurodevelopmental genes, such as OTX2 and OLIG2. Monoallelic genes also showed increased levels of DNA methylation compared to hypomethylated biallelic loci. Identified monoallelic gene loci showed altered chromatin signatures in fetal brain, suggesting an in vivo correlate of this phenomenon. We conclude that stochastic allelic expression is prevalent in neural stem cells, providing clonal diversity to developing tissues such as the human brain.",
author = "Jeffries, {Aaron R.} and Leo Perfect and Julia Ledderose and Schalkwyk, {Leonard C.} and Bray, {Nicholas J.} and Jonathan Mill and Jack Price",
year = "2012",
month = sep,
doi = "10.1002/stem.1155",
language = "English",
volume = "30",
pages = "1938--1947",
journal = "Stem Cells",
issn = "1066-5099",
publisher = "Nova Science Publishers Inc",
number = "9",

}

RIS (suitable for import to EndNote) Download

TY - JOUR

T1 - Stochastic Choice of Allelic Expression in Human Neural Stem Cells

AU - Jeffries, Aaron R.

AU - Perfect, Leo

AU - Ledderose, Julia

AU - Schalkwyk, Leonard C.

AU - Bray, Nicholas J.

AU - Mill, Jonathan

AU - Price, Jack

PY - 2012/9

Y1 - 2012/9

N2 - Monoallelic gene expression, such as genomic imprinting, is well described. Less well-characterized are genes undergoing stochastic monoallelic expression (MA), where specific clones of cells express just one allele at a given locus. We performed genome-wide allelic expression assessment of human clonal neural stem cells derived from cerebral cortex, striatum, and spinal cord, each with differing genotypes. We assayed three separate clonal lines from each donor, distinguishing stochastic MA from genotypic effects. Roughly 2% of genes showed evidence for autosomal MA, and in about half of these, allelic expression was stochastic between different clones. Many of these loci were known neurodevelopmental genes, such as OTX2 and OLIG2. Monoallelic genes also showed increased levels of DNA methylation compared to hypomethylated biallelic loci. Identified monoallelic gene loci showed altered chromatin signatures in fetal brain, suggesting an in vivo correlate of this phenomenon. We conclude that stochastic allelic expression is prevalent in neural stem cells, providing clonal diversity to developing tissues such as the human brain.

AB - Monoallelic gene expression, such as genomic imprinting, is well described. Less well-characterized are genes undergoing stochastic monoallelic expression (MA), where specific clones of cells express just one allele at a given locus. We performed genome-wide allelic expression assessment of human clonal neural stem cells derived from cerebral cortex, striatum, and spinal cord, each with differing genotypes. We assayed three separate clonal lines from each donor, distinguishing stochastic MA from genotypic effects. Roughly 2% of genes showed evidence for autosomal MA, and in about half of these, allelic expression was stochastic between different clones. Many of these loci were known neurodevelopmental genes, such as OTX2 and OLIG2. Monoallelic genes also showed increased levels of DNA methylation compared to hypomethylated biallelic loci. Identified monoallelic gene loci showed altered chromatin signatures in fetal brain, suggesting an in vivo correlate of this phenomenon. We conclude that stochastic allelic expression is prevalent in neural stem cells, providing clonal diversity to developing tissues such as the human brain.

U2 - 10.1002/stem.1155

DO - 10.1002/stem.1155

M3 - Article

VL - 30

SP - 1938

EP - 1947

JO - Stem Cells

JF - Stem Cells

SN - 1066-5099

IS - 9

ER -

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