Stochastic patterns in globin gene expression are established prior to transcriptional activation and are clonally inherited

Mariken de Krom; Mariette van de Corput; Marieke von Lindern; Frank Grosveld; John Strouboulis

doi:10.1016/s1097-2765(02)00558-0

Stochastic patterns in globin gene expression are established prior to transcriptional activation and are clonally inherited

Mariken de Krom, Mariette van de Corput, Marieke von Lindern, Frank Grosveld, John Strouboulis

Comprehensive Cancer Centre

Department of Epidemiology, Erasmus University Rotterdam, University Medical Centre Rotterdam, Rotterdam, the Netherlands.

Research output: Contribution to journal › Article › peer-review

50 Citations (Scopus)

Abstract

We have undertaken a detailed characterization of mouse globin gene expression patterns in the nucleus and cytoplasm of single erythroid cells. We demonstrate an imbalance of alpha- versus beta-globin expression in a significant proportion of cells both in nuclear transcription patterns and cytoplasmic mRNA levels. Clonal cell analysis showed these expression patterns to be clonally inherited, while analysis of a multicopy transgenic locus showed an all-or-none effect in the activation of all the genes in one locus. These data provide strong evidence for a stochastic basis of globin gene activation resulting in heritable all-or-none expression patterns.

Original language	English
Pages (from-to)	1319-26
Number of pages	8
Journal	MOLECULAR CELL
Volume	9
Issue number	6
DOIs	https://doi.org/10.1016/s1097-2765(02)00558-0
Publication status	Published - Jun 2002

Keywords

Animals
Cell Differentiation/physiology
Cell Fractionation
Erythrocytes/cytology
Gene Expression Regulation
Globins/genetics
Hepatocytes/cytology
Humans
In Situ Hybridization
Mice
Mice, Transgenic
RNA, Messenger/metabolism
Transcriptional Activation

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10.1016/s1097-2765(02)00558-0

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title = "Stochastic patterns in globin gene expression are established prior to transcriptional activation and are clonally inherited",

abstract = "We have undertaken a detailed characterization of mouse globin gene expression patterns in the nucleus and cytoplasm of single erythroid cells. We demonstrate an imbalance of alpha- versus beta-globin expression in a significant proportion of cells both in nuclear transcription patterns and cytoplasmic mRNA levels. Clonal cell analysis showed these expression patterns to be clonally inherited, while analysis of a multicopy transgenic locus showed an all-or-none effect in the activation of all the genes in one locus. These data provide strong evidence for a stochastic basis of globin gene activation resulting in heritable all-or-none expression patterns.",

keywords = "Animals, Cell Differentiation/physiology, Cell Fractionation, Erythrocytes/cytology, Gene Expression Regulation, Globins/genetics, Hepatocytes/cytology, Humans, In Situ Hybridization, Mice, Mice, Transgenic, RNA, Messenger/metabolism, Transcriptional Activation",

author = "{de Krom}, Mariken and {van de Corput}, Mariette and {von Lindern}, Marieke and Frank Grosveld and John Strouboulis",

year = "2002",

month = jun,

doi = "10.1016/s1097-2765(02)00558-0",

language = "English",

volume = "9",

pages = "1319--26",

journal = "MOLECULAR CELL",

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publisher = "Cell Press",

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TY - JOUR

T1 - Stochastic patterns in globin gene expression are established prior to transcriptional activation and are clonally inherited

AU - de Krom, Mariken

AU - van de Corput, Mariette

AU - von Lindern, Marieke

AU - Grosveld, Frank

AU - Strouboulis, John

PY - 2002/6

Y1 - 2002/6

N2 - We have undertaken a detailed characterization of mouse globin gene expression patterns in the nucleus and cytoplasm of single erythroid cells. We demonstrate an imbalance of alpha- versus beta-globin expression in a significant proportion of cells both in nuclear transcription patterns and cytoplasmic mRNA levels. Clonal cell analysis showed these expression patterns to be clonally inherited, while analysis of a multicopy transgenic locus showed an all-or-none effect in the activation of all the genes in one locus. These data provide strong evidence for a stochastic basis of globin gene activation resulting in heritable all-or-none expression patterns.

AB - We have undertaken a detailed characterization of mouse globin gene expression patterns in the nucleus and cytoplasm of single erythroid cells. We demonstrate an imbalance of alpha- versus beta-globin expression in a significant proportion of cells both in nuclear transcription patterns and cytoplasmic mRNA levels. Clonal cell analysis showed these expression patterns to be clonally inherited, while analysis of a multicopy transgenic locus showed an all-or-none effect in the activation of all the genes in one locus. These data provide strong evidence for a stochastic basis of globin gene activation resulting in heritable all-or-none expression patterns.

KW - Animals

KW - Cell Differentiation/physiology

KW - Cell Fractionation

KW - Erythrocytes/cytology

KW - Gene Expression Regulation

KW - Globins/genetics

KW - Hepatocytes/cytology

KW - Humans

KW - In Situ Hybridization

KW - Mice

KW - Mice, Transgenic

KW - RNA, Messenger/metabolism

KW - Transcriptional Activation

U2 - 10.1016/s1097-2765(02)00558-0

DO - 10.1016/s1097-2765(02)00558-0

M3 - Article

C2 - 12086628

SN - 1097-2765

VL - 9

SP - 1319

EP - 1326

JO - MOLECULAR CELL

JF - MOLECULAR CELL

IS - 6

ER -

Stochastic patterns in globin gene expression are established prior to transcriptional activation and are clonally inherited

Abstract

Keywords

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