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STRSeq: A catalog of sequence diversity at human identification Short Tandem Repeat loci

Research output: Contribution to journalArticle

Katherine Butler Gettings, Lisa A. Borsuk, David Ballard, Martin Bodner, Bruce Budowle, Laurence Devesse, Jonathan King, Walther Parson, Christopher Phillips, Peter M. Vallone

Original languageEnglish
Pages (from-to)111-117
Number of pages7
JournalForensic Science International-Genetics
Volume31
Early online date1 Sep 2017
DOIs
Accepted/In press30 Aug 2017
E-pub ahead of print1 Sep 2017

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Abstract

The STR Sequencing Project (STRSeq) was initiated to facilitate the description of sequence-based alleles at the Short Tandem Repeat (STR) loci targeted in human identification assays. This international collaborative effort, which has been endorsed by the ISFG DNA Commission, provides a framework for communication among laboratories. The initial data used to populate the project are the aggregate alleles observed in targeted sequencing studies across four laboratories: National Institute of Standards and Technology (N = 1786), Kings College London (N = 1043), University of North Texas Health Sciences Center (N = 839), and University of Santiago de Compostela (N = 944), for a total of 4612 individuals. STRSeq data are maintained as GenBank records at the U.S. National Center for Biotechnology Information (NCBI), which participates in a daily data exchange with the DNA DataBank of Japan (DDBJ) and the European Nucleotide Archive (ENA). Each GenBank record contains the observed sequence of a STR region, annotation (“bracketing”) of the repeat region and flanking region polymorphisms, information regarding the sequencing assay and data quality, and backward compatible length-based allele designation. STRSeq GenBank records are organized within a BioProject at NCBI (https://www.ncbi.nlm.nih.gov/bioproject/380127), which is sub-divided into: commonly used autosomal STRs, alternate autosomal STRs, Y-chromosomal STRs, and X-chromosomal STRs. Each of these categories is further divided into locus-specific BioProjects. The BioProject hierarchy facilitates access to the GenBank records by browsing, BLAST searching, or ftp download. Future plans include user interface tools at strseq.nist.gov, a pathway for submission of additional allele records by laboratories performing population sample sequencing and interaction with the STRidER web portal for quality control (http://strider.online).

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