Structural Analysis of Bacillus subtilis Sigma Factors

TY - JOUR

T1 - Structural Analysis of Bacillus subtilis Sigma Factors

AU - Collins, Katherine M.

AU - Evans, Nicola J.

AU - Torpey, James H.

AU - Harris, Jonathon M.

AU - Haynes, Bethany A.

AU - Camp, Amy H.

AU - Isaacson, Rivka L.

N1 - Funding Information: RLI’s work on Bacillus subtilis has been funded by BBSRC grants: BB/N006267/1; BB/R006091/1; BB/S006877/1. AHC was supported by National Institutes of Health grants DP2 GM105439 and R15 GM101559. The authors would like to thank Diamond Light Source for beamtime (proposal mx13597; King’s College London BAG). NMR experiments were performed at the Centre for Biomolecular Spectroscopy, King’s College London, established with a Capital Award from the Wellcome Trust. This work was supported by the Francis Crick Institute through provision of access to the MRC Biomedical NMR Centre. The Francis Crick Institute received core funding from Cancer Research UK (FC001029), the UK Medical Research Council (FC001029), and the Wellcome Trust (FC001029). The 950 MHz NMR facility at the University of Oxford was funded by the Wellcome Trust Joint Infrastructure Fund and the E. P. Abraham Fund. Publisher Copyright: © 2023 by the authors.

PY - 2023/4/20

Y1 - 2023/4/20

N2 - Bacteria use an array of sigma factors to regulate gene expression during different stages of their life cycles. Full-length, atomic-level structures of sigma factors have been challenging to obtain experimentally as a result of their many regions of intrinsic disorder. AlphaFold has now supplied plausible full-length models for most sigma factors. Here we discuss the current understanding of the structures and functions of sigma factors in the model organism, Bacillus subtilis, and present an X-ray crystal structure of a region of B. subtilis SigE, a sigma factor that plays a critical role in the developmental process of spore formation.

AB - Bacteria use an array of sigma factors to regulate gene expression during different stages of their life cycles. Full-length, atomic-level structures of sigma factors have been challenging to obtain experimentally as a result of their many regions of intrinsic disorder. AlphaFold has now supplied plausible full-length models for most sigma factors. Here we discuss the current understanding of the structures and functions of sigma factors in the model organism, Bacillus subtilis, and present an X-ray crystal structure of a region of B. subtilis SigE, a sigma factor that plays a critical role in the developmental process of spore formation.

KW - Alphafold

KW - Bacillus subtilis

KW - SigE

KW - sigma factor

KW - sporulation

KW - structure

KW - X-ray crystallography

UR - http://www.scopus.com/inward/record.url?scp=85156206694&partnerID=8YFLogxK

U2 - 10.3390/microorganisms11041077

DO - 10.3390/microorganisms11041077

M3 - Article

AN - SCOPUS:85156206694

SN - 2076-2607

VL - 11

JO - Microorganisms

JF - Microorganisms

IS - 4

M1 - 1077

ER -