Structural characterisation of medically relevant protein assemblies by integrating mass spectrometry with computational modelling

Argyris Politis; Carla Schmidt

doi:10.1016/j.jprot.2017.04.019

Structural characterisation of medically relevant protein assemblies by integrating mass spectrometry with computational modelling

Argyris Politis^*, Carla Schmidt

^*Corresponding author for this work

Chemistry

University of Halle

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

238 Downloads (Pure)

Abstract

Structural mass spectrometry with its various techniques is a powerful tool for the structural elucidation of medically relevant protein assemblies. It delivers information on the composition, stoichiometries, interactions and topologies of these assemblies. Most importantly it can deal with heterogeneous mixtures and assemblies which makes it universal among the conventional structural techniques. In this review we summarise recent advances and challenges in structural mass spectrometric techniques. We describe how the combination of the different mass spectrometry-based methods with computational strategies enable structural models at molecular levels of resolution. These models hold significant potential for helping us in characterizing the function of protein assemblies related to human health and disease.

Original language	English
Journal	Journal Of Proteomics
Early online date	28 Apr 2017
DOIs	https://doi.org/10.1016/j.jprot.2017.04.019
Publication status	E-pub ahead of print - 28 Apr 2017

Keywords

Computational modelling
Integrative modelling
Proteomics
Structural mass spectrometry

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AB - Structural mass spectrometry with its various techniques is a powerful tool for the structural elucidation of medically relevant protein assemblies. It delivers information on the composition, stoichiometries, interactions and topologies of these assemblies. Most importantly it can deal with heterogeneous mixtures and assemblies which makes it universal among the conventional structural techniques. In this review we summarise recent advances and challenges in structural mass spectrometric techniques. We describe how the combination of the different mass spectrometry-based methods with computational strategies enable structural models at molecular levels of resolution. These models hold significant potential for helping us in characterizing the function of protein assemblies related to human health and disease.

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KW - Integrative modelling

KW - Proteomics

KW - Structural mass spectrometry

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Structural characterisation of medically relevant protein assemblies by integrating mass spectrometry with computational modelling

Abstract

Keywords

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