Structural Modeling of T9SS Outer Membrane Proteins and Their Complexes

Research output: Chapter in Book/Report/Conference proceedingChapter


The type 9 secretion system (T9SS) is a recently discovered machinery that both transports cargo proteins across the Gram-negative bacterial outer membrane and attaches them to lipopolysaccharides on the extracellular surface. Outer membrane proteins (OMPs) are key components of the T9SS and are involved in both steps. In this chapter, we describe a method for the in silico modeling of T9SS OMPs and their complexes, and model validation. This is useful when the production of recombinant OMPs is difficult, and these protocols can also be applied to OMP complexes outside of the T9SS.

Original languageEnglish
Title of host publicationTransmembrane β-Barrel Proteins
Subtitle of host publicationMethods and Protocols
Number of pages14
Publication statusPublished - 14 Mar 2024

Publication series

NameMethods in molecular biology (Clifton, N.J.)
ISSN (Print)1064-3745


  • Membrane Proteins
  • Bacterial Outer Membrane Proteins/metabolism
  • Bacterial Proteins/metabolism


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