Structure and Genetic Analysis of the Arterivirus Nonstructural Protein 7 alpha

Ioannis Manolaridis; Cyril Gaudin; Clara C. Posthuma; Jessika C. Zevenhoven-Dobbe; Isabelle Imbert; Bruno Canard; Geoff Kelly; Paul A. Tucker; Maria R. Conte; Eric J. Snijder

doi:10.1128/JVI.00255-11

Structure and Genetic Analysis of the Arterivirus Nonstructural Protein 7 alpha

Ioannis Manolaridis
, Cyril Gaudin
, Clara C. Posthuma
, Jessika C. Zevenhoven-Dobbe
, Isabelle Imbert
, Bruno Canard
, Geoff Kelly
, Paul A. Tucker
, Maria R. Conte
, Eric J. Snijder

European Mol Biol Lab, Hamburg Outstn
LUMC Leiden University Medical Center
CNRS Centre National de la Recerche Scientifique
Aix-Marseille University
Natl Inst Med Res, Biomed NMR Ctr

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Arterivirus replicase polyproteins are cleaved into at least 13 mature nonstructural proteins (nsps), and in particular the nsp5-to-nsp8 region is subject to a complex processing cascade. The function of the largest subunit from this region, nsp7, which is further cleaved into nsp7 alpha and nsp7 beta, is unknown. Using nuclear magnetic resonance (NMR) spectroscopy, we determined the solution structure of nsp7 alpha of equine arteritis virus, revealing an interesting unique fold for this protein but thereby providing little clue to its possible functions. Nevertheless, structure-based reverse genetics studies established the importance of nsp7/nsp7 alpha for viral RNA synthesis, thus providing a basis for future studies.

Original language	English
Pages (from-to)	7449 - 7453
Number of pages	5
Journal	Journal of Virology
Volume	85
Issue number	14
DOIs	https://doi.org/10.1128/JVI.00255-11
Publication status	Published - Jul 2011

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title = "Structure and Genetic Analysis of the Arterivirus Nonstructural Protein 7 alpha",

abstract = "Arterivirus replicase polyproteins are cleaved into at least 13 mature nonstructural proteins (nsps), and in particular the nsp5-to-nsp8 region is subject to a complex processing cascade. The function of the largest subunit from this region, nsp7, which is further cleaved into nsp7 alpha and nsp7 beta, is unknown. Using nuclear magnetic resonance (NMR) spectroscopy, we determined the solution structure of nsp7 alpha of equine arteritis virus, revealing an interesting unique fold for this protein but thereby providing little clue to its possible functions. Nevertheless, structure-based reverse genetics studies established the importance of nsp7/nsp7 alpha for viral RNA synthesis, thus providing a basis for future studies.",

author = "Ioannis Manolaridis and Cyril Gaudin and Posthuma, \{Clara C.\} and Zevenhoven-Dobbe, \{Jessika C.\} and Isabelle Imbert and Bruno Canard and Geoff Kelly and Tucker, \{Paul A.\} and Conte, \{Maria R.\} and Snijder, \{Eric J.\}",

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T1 - Structure and Genetic Analysis of the Arterivirus Nonstructural Protein 7 alpha

AU - Manolaridis, Ioannis

AU - Gaudin, Cyril

AU - Posthuma, Clara C.

AU - Zevenhoven-Dobbe, Jessika C.

AU - Imbert, Isabelle

AU - Canard, Bruno

AU - Kelly, Geoff

AU - Tucker, Paul A.

AU - Conte, Maria R.

AU - Snijder, Eric J.

PY - 2011/7

Y1 - 2011/7

N2 - Arterivirus replicase polyproteins are cleaved into at least 13 mature nonstructural proteins (nsps), and in particular the nsp5-to-nsp8 region is subject to a complex processing cascade. The function of the largest subunit from this region, nsp7, which is further cleaved into nsp7 alpha and nsp7 beta, is unknown. Using nuclear magnetic resonance (NMR) spectroscopy, we determined the solution structure of nsp7 alpha of equine arteritis virus, revealing an interesting unique fold for this protein but thereby providing little clue to its possible functions. Nevertheless, structure-based reverse genetics studies established the importance of nsp7/nsp7 alpha for viral RNA synthesis, thus providing a basis for future studies.

AB - Arterivirus replicase polyproteins are cleaved into at least 13 mature nonstructural proteins (nsps), and in particular the nsp5-to-nsp8 region is subject to a complex processing cascade. The function of the largest subunit from this region, nsp7, which is further cleaved into nsp7 alpha and nsp7 beta, is unknown. Using nuclear magnetic resonance (NMR) spectroscopy, we determined the solution structure of nsp7 alpha of equine arteritis virus, revealing an interesting unique fold for this protein but thereby providing little clue to its possible functions. Nevertheless, structure-based reverse genetics studies established the importance of nsp7/nsp7 alpha for viral RNA synthesis, thus providing a basis for future studies.

U2 - 10.1128/JVI.00255-11

DO - 10.1128/JVI.00255-11

M3 - Article

SN - 1098-5514

VL - 85

SP - 7449

EP - 7453

JO - Journal of Virology

JF - Journal of Virology

IS - 14

ER -

Structure and Genetic Analysis of the Arterivirus Nonstructural Protein 7 alpha

Abstract

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