Structure of a quinolone-stabilized cleavage complex of topoisomerase IV from it Klebsiella pneumoniae and comparison with a related it Streptococcus pneumoniae complex

Dennis A. Veselkov, Ivan Laponogov, Xiao-Su Pan, Jogitha Selvarajah, Galyna B. Skamrova, Arthur Branstrom, Jana Narasimhan, Josyula V. N. Vara Prasad, L. Mark Fisher, Mark R. Sanderson

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31 Citations (Scopus)
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Abstract

Klebsiella pneumoniae is a Gram-negative bacterium that is responsible for a range of common infections, including pulmonary pneumonia, bloodstream infections and meningitis. Certain strains of it Klebsiella have become highly resistant to antibiotics. Despite the vast amount of research carried out on this class of bacteria, the molecular structure of its topoisomerase IV, a type II topoisomerase essential for catalysing chromosomal segregation, had remained unknown. In this paper, the structure of its DNA-cleavage complex is reported at 3.35Å resolution. The complex is comprised of ParC breakage-reunion and ParE TOPRIM domains of it K. pneumoniae topoisomerase IV with DNA stabilized by levofloxacin, a broad-spectrum fluoroquinolone antimicrobial agent. This complex is compared with a similar complex from it Streptococcus pneumoniae, which has recently been solved.
Original languageEnglish
Pages (from-to)488-496
Number of pages9
JournalActa Crystallographica Section D Structural Biology
Volume72
Issue number4
Early online date24 Mar 2016
DOIs
Publication statusPublished - 2016

Keywords

  • Klebsiella pneumoniae, cleavage complex, quinolone, levofloxacin, topoisomerase IV, DNA binding, isomerase, isomerase-DNA complex, topoisomerases, Gram-negative complexes, X-ray crystallography, protein-DNA-drug complexes

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