Substrate evaluation for a microbeam endstation using unstained cell imaging

G. Flaccavento; M. Folkard; J. A. Noble; K. M. Prise; Boris Vojnovic

doi:10.1016/j.apradiso.2008.06.017

Substrate evaluation for a microbeam endstation using unstained cell imaging

G. Flaccavento^*, M. Folkard, J. A. Noble, K. M. Prise, Boris Vojnovic

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

A cellular imaging system, optimized for unstained cells seeded onto a thin substrate, is under development. This system will be a component of the endstation for the microbeam cell-irradiation facility at the University of Surrey. Previous irradiation experiments at the Gray Cancer Institute (GCI) have used Mylar (TM) film to support the cells [Folkard, M., Prise, K,, Schettino, G., Shao, C., Gilchrist, S., Vojnovic, B., 2005. New insights into the cellular response to radiation using microbeams. Nucl. Instrum. Methods B 231, 189-194]. Although suitable for fluorescence microscopy, the Mylar (TM) often creates excessive optical noise when used with non-fluorescent microscopy. A variety of substrates are being investigated to provide appropriate optical clarity, cell adhesion, and radiation attenuation. This paper reports on our investigations to date.

Original language	English
Article number	N/A
Pages (from-to)	460-463
Number of pages	4
Journal	Applied Radiation and Isotopes
Volume	67
Issue number	3
DOIs	https://doi.org/10.1016/j.apradiso.2008.06.017
Publication status	Published - Mar 2009

Keywords

Microbeam
Substrate
Cell dish
Single-cell irradiation
Phase contrast
Polymer
IRRADIATION
PARTICLES

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.apradiso.2008.06.017

Cite this

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title = "Substrate evaluation for a microbeam endstation using unstained cell imaging",

abstract = "A cellular imaging system, optimized for unstained cells seeded onto a thin substrate, is under development. This system will be a component of the endstation for the microbeam cell-irradiation facility at the University of Surrey. Previous irradiation experiments at the Gray Cancer Institute (GCI) have used Mylar (TM) film to support the cells [Folkard, M., Prise, K,, Schettino, G., Shao, C., Gilchrist, S., Vojnovic, B., 2005. New insights into the cellular response to radiation using microbeams. Nucl. Instrum. Methods B 231, 189-194]. Although suitable for fluorescence microscopy, the Mylar (TM) often creates excessive optical noise when used with non-fluorescent microscopy. A variety of substrates are being investigated to provide appropriate optical clarity, cell adhesion, and radiation attenuation. This paper reports on our investigations to date.",

keywords = "Microbeam, Substrate, Cell dish, Single-cell irradiation, Phase contrast, Polymer, IRRADIATION, PARTICLES",

author = "G. Flaccavento and M. Folkard and Noble, {J. A.} and Prise, {K. M.} and Boris Vojnovic",

year = "2009",

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doi = "10.1016/j.apradiso.2008.06.017",

language = "English",

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journal = "Applied Radiation and Isotopes",

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AU - Flaccavento, G.

AU - Folkard, M.

AU - Noble, J. A.

AU - Prise, K. M.

AU - Vojnovic, Boris

PY - 2009/3

Y1 - 2009/3

N2 - A cellular imaging system, optimized for unstained cells seeded onto a thin substrate, is under development. This system will be a component of the endstation for the microbeam cell-irradiation facility at the University of Surrey. Previous irradiation experiments at the Gray Cancer Institute (GCI) have used Mylar (TM) film to support the cells [Folkard, M., Prise, K,, Schettino, G., Shao, C., Gilchrist, S., Vojnovic, B., 2005. New insights into the cellular response to radiation using microbeams. Nucl. Instrum. Methods B 231, 189-194]. Although suitable for fluorescence microscopy, the Mylar (TM) often creates excessive optical noise when used with non-fluorescent microscopy. A variety of substrates are being investigated to provide appropriate optical clarity, cell adhesion, and radiation attenuation. This paper reports on our investigations to date.

AB - A cellular imaging system, optimized for unstained cells seeded onto a thin substrate, is under development. This system will be a component of the endstation for the microbeam cell-irradiation facility at the University of Surrey. Previous irradiation experiments at the Gray Cancer Institute (GCI) have used Mylar (TM) film to support the cells [Folkard, M., Prise, K,, Schettino, G., Shao, C., Gilchrist, S., Vojnovic, B., 2005. New insights into the cellular response to radiation using microbeams. Nucl. Instrum. Methods B 231, 189-194]. Although suitable for fluorescence microscopy, the Mylar (TM) often creates excessive optical noise when used with non-fluorescent microscopy. A variety of substrates are being investigated to provide appropriate optical clarity, cell adhesion, and radiation attenuation. This paper reports on our investigations to date.

KW - Microbeam

KW - Substrate

KW - Cell dish

KW - Single-cell irradiation

KW - Phase contrast

KW - Polymer

KW - IRRADIATION

KW - PARTICLES

U2 - 10.1016/j.apradiso.2008.06.017

DO - 10.1016/j.apradiso.2008.06.017

M3 - Article

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EP - 463

JO - Applied Radiation and Isotopes

JF - Applied Radiation and Isotopes

IS - 3

M1 - N/A

ER -

Substrate evaluation for a microbeam endstation using unstained cell imaging

Abstract

Keywords

UN SDGs

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