Suppression of topoisomerase IIalpha expression and function in human cells decreases chromosomal radiosensitivity

Samantha Y A Terry, Andrew C Riches, Peter E Bryant

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

The mechanism behind chromatid break formation is as yet unclear, although it is known that DNA double-strand breaks (DSBs) are the initiating lesions. Chromatid breaks formed in cells in the G2-phase of the cell-cycle disappear ('rejoin') as a function of time between radiation exposure and cell fixation. However, the kinetics of disappearance of chromatid breaks does not correspond to those of DSB rejoining, leading us to seek alternative models. We have proposed that chromatid breaks could be formed indirectly from DSB and that the mechanism involves topoisomerase IIalpha. In support of this hypothesis we have recently shown that frequencies of radiation-induced chromatid breaks are lower in two variant human promyelocytic leukaemic cell lines with reduced topoisomerase IIalpha expression. Here we report that suppression of topoisomerase IIalpha in human hTERT-RPE1 cells, either by its abrogation using specific siRNA or by inhibition of its catalytic activity with the inhibitor ICRF-193, causes a reduction in frequency of chromatid breaks in radiation-exposed cells. The findings support our hypothesis for the involvement of topoisomerase IIalpha in the formation of radiation-induced chromatid breaks, and could help explain inter-individual variation in human chromosomal radiosensitivity; elevation of which has been linked with cancer susceptibility.

Original languageEnglish
Pages (from-to)40-5
Number of pages6
JournalMutation Research
Volume663
Issue number1-2
DOIs
Publication statusPublished - 26 Apr 2009

Keywords

  • Antigens, Neoplasm
  • Blotting, Western
  • Cell Line
  • Chromatids
  • Chromosomes, Human
  • DNA Topoisomerases, Type II
  • DNA-Binding Proteins
  • Gamma Rays
  • Humans
  • Immunoblotting
  • Immunohistochemistry
  • Mitotic Index
  • Piperazines
  • RNA, Messenger
  • RNA, Small Interfering
  • Radiation Tolerance
  • Topoisomerase II Inhibitors
  • Transcription, Genetic

Fingerprint

Dive into the research topics of 'Suppression of topoisomerase IIalpha expression and function in human cells decreases chromosomal radiosensitivity'. Together they form a unique fingerprint.

Cite this